Enzyme synergies in the hydrolysis of Afex pretreated biomass

Bruce Dale, Shishir P.S. Chundawat, Balan Venkatesh

Research output: Contribution to conferencePaperpeer-review


The concept of synergistic enzymatic hydrolysis of cellulosic biomass has been extant for the past fifty years. Much research has been dedicated to studying the synergy between various cellulase enzymes on cellulose. However, little work has been directed to understanding the nature of synergy between cellulases, hemicellulases and other accessory enzymes on complex lignocellulosic substrates. In addition, most past research has focused on pretreatment technologies, such as acid hydrolysis, that emphasize recovering hemicellulose-derived sugars in the chemical pretreatment step itself. With additional attention now being paid to alkaline pretreatments such as ammonia fiber explosion (AFEX) technology, the need to develop efficient enzyme systems that hydrolyze the xylans as well as the glucans becomes apparent. The efficacy by which these mixed enzyme systems hydrolyze the biomass substrate is inextricably linked to the innate structural characteristics (on multiple structural levels) of the AFEX pretreated substrate and enzyme related factors as well. In these results, we attempt to understand the role of AFEX pretreatment on enzyme synergy and to optimize the tailored mixture of hemicellulases/cellulases that are necessary and sufficient for biomass conversion. The ultimate goal is to develop economic process options effective in producing high sugar yields at the lowest enzyme loading (cellulase plus hemicellulase).

Original languageEnglish (US)
Number of pages1
StatePublished - 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005


Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
Country/TerritoryUnited States
CityCincinnati, OH

All Science Journal Classification (ASJC) codes

  • Engineering(all)


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