Escherichia coli DNA topoisomerase I mutants: Increased supercoiling is corrected by mutations near gyrase genes

Gail J. Pruss; Stephen H. Manes; Karl Drlica

doi:10.1016/0092-8674(82)90402-0

Escherichia coli DNA topoisomerase I mutants: Increased supercoiling is corrected by mutations near gyrase genes

Gail J. Pruss, Stephen H. Manes, Karl Drlica

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Abstract

Bacterial chromosomes and plasmid (pBR322) DNA from topoisomerase I-defective Escherichia coli strains have been characterized with respect to superhelical density. The topoisomerase I defect results in increased negative superhelical density of both the bacterial chromosome and pBR322. Thus topoisomerase I is involved in determining the level of supercoiling in bacteria. Three of the topoisomerase I-defective strains we studied carry secondary mutations that decrease superhelical density; these additional mutations are closely linked to the gyrB locus in two of the strains and to the gyrA locus in the third strain.

Original language	English (US)
Pages (from-to)	35-42
Number of pages	8
Journal	Cell
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(82)90402-0
State	Published - Nov 1982
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(82)90402-0

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@article{752fb4a489fa43c8839dba8c493cbd72,

title = "Escherichia coli DNA topoisomerase I mutants: Increased supercoiling is corrected by mutations near gyrase genes",

abstract = "Bacterial chromosomes and plasmid (pBR322) DNA from topoisomerase I-defective Escherichia coli strains have been characterized with respect to superhelical density. The topoisomerase I defect results in increased negative superhelical density of both the bacterial chromosome and pBR322. Thus topoisomerase I is involved in determining the level of supercoiling in bacteria. Three of the topoisomerase I-defective strains we studied carry secondary mutations that decrease superhelical density; these additional mutations are closely linked to the gyrB locus in two of the strains and to the gyrA locus in the third strain.",

author = "Pruss, {Gail J.} and Manes, {Stephen H.} and Karl Drlica",

note = "Funding Information: We thank Rolf Sternglanz and Stephen DlNardo for generously pro-vlding bacterial strains and communicating unpublished results. We also thank James Tricoli and David Kowalski for providing topoiso-merase I. We are grateful to Joanna Olmsted, Lasse Lindahl, Janice Zengel, Len Rosenberg, Thomas Krugh and Carl Rosenfeld for many helpful discussions. This work was supported by a grant from the U. S. Public Health Service, and by a Research Career Development Award (to K. 0.) from the National Cancer Institute.",

year = "1982",

month = nov,

doi = "10.1016/0092-8674(82)90402-0",

language = "English (US)",

volume = "31",

pages = "35--42",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Escherichia coli DNA topoisomerase I mutants

T2 - Increased supercoiling is corrected by mutations near gyrase genes

AU - Pruss, Gail J.

AU - Manes, Stephen H.

AU - Drlica, Karl

N1 - Funding Information: We thank Rolf Sternglanz and Stephen DlNardo for generously pro-vlding bacterial strains and communicating unpublished results. We also thank James Tricoli and David Kowalski for providing topoiso-merase I. We are grateful to Joanna Olmsted, Lasse Lindahl, Janice Zengel, Len Rosenberg, Thomas Krugh and Carl Rosenfeld for many helpful discussions. This work was supported by a grant from the U. S. Public Health Service, and by a Research Career Development Award (to K. 0.) from the National Cancer Institute.

PY - 1982/11

Y1 - 1982/11

N2 - Bacterial chromosomes and plasmid (pBR322) DNA from topoisomerase I-defective Escherichia coli strains have been characterized with respect to superhelical density. The topoisomerase I defect results in increased negative superhelical density of both the bacterial chromosome and pBR322. Thus topoisomerase I is involved in determining the level of supercoiling in bacteria. Three of the topoisomerase I-defective strains we studied carry secondary mutations that decrease superhelical density; these additional mutations are closely linked to the gyrB locus in two of the strains and to the gyrA locus in the third strain.

AB - Bacterial chromosomes and plasmid (pBR322) DNA from topoisomerase I-defective Escherichia coli strains have been characterized with respect to superhelical density. The topoisomerase I defect results in increased negative superhelical density of both the bacterial chromosome and pBR322. Thus topoisomerase I is involved in determining the level of supercoiling in bacteria. Three of the topoisomerase I-defective strains we studied carry secondary mutations that decrease superhelical density; these additional mutations are closely linked to the gyrB locus in two of the strains and to the gyrA locus in the third strain.

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VL - 31

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JO - Cell

JF - Cell

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ER -

Escherichia coli DNA topoisomerase I mutants: Increased supercoiling is corrected by mutations near gyrase genes

Abstract

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