Establishment of clones of SM22α-EGFP expressing embryonic stem cells and in vitro dynamic tracing of smooth muscle cells development

Ya Ling Han, Kai Xu, Jian Kang, Cheng Hui Yan, Xiao Xiang Tian, Shao Hua Li

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A murine embryonic stem cell (ESC) line stably expressing the enhanced green fluorescent protein (EGFP) under the transcriptional control of the smooth-muscle-specific SM22α promoter to further characterize development of the vascular smooth muscle cells (VSMCs) differentiated from ESCs is established. In SM22α-EGFP expressing ESC-derived embryoid bodies(EBs), a distinct sublineage of VSMCs could be identified by EGFP fluorescence. The SM22α promoter was switched on at day 11, and EGFP-positive cells increased gradually and reached peak at day 30. The specificity of EGFP positive cells was corroborated by RT-PCR analysis and immunostaining with antibodies against known markers for VSMCs. VSMCs were heterogeneous in their morphology in plating EBs, and could be divided into two categories: spindle-shaped or epithelioid, polygonal cells. These results suggest that SM22α-EGFP expression enables the identification of ESC -derived VSMCs by their fluorescence and morphology.

Original languageEnglish (US)
Pages (from-to)343-349
Number of pages7
JournalProgress in Biochemistry and Biophysics
Volume33
Issue number4
StatePublished - Apr 2006

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Keywords

  • Differentiation
  • Embryonic stem cell
  • Migration
  • Smooth muscle cell

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