Establishment of Polycomb silencing requires a transient interaction between PC and ESC

S. Poux, R. Melfi, V. Pirrotta

Research output: Contribution to journalArticlepeer-review

138 Scopus citations

Abstract

Two distinct types of Polycomb complexes have been identified in flies and in vertebrates, one containing ESC and one containing PC. Using LexA fusions, we show that PC and ESC can establish silencing of a reporter gene but that each requires the presence of the other. In early embryonic extracts, we find PC transiently associated with ESC in a complex that includes EZ, PHO, PH, GAGA, and RPD3 but not PSC. In older embryos, PC is found in a complex including PH, PSC, GAGA, and RPD3, whereas ESC is in a separate complex including EZ, PHO, and RPD3.

Original languageEnglish (US)
Pages (from-to)2509-2514
Number of pages6
JournalGenes and Development
Volume15
Issue number19
DOIs
StatePublished - Oct 1 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • ESC/PHO
  • PcG complex
  • Polycomb silencing

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