Estimation of 2n megagametophyte heterozygosity in a diploid blueberry (Vaccinium darrowi Camp) clone using RAPDs

N. Vorsa, L. J. Rowland

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Abstract

To determine the mode(s) of 2n gamete formation in a Vaccinium darrowi Camp (2n = 2x = 24) clone Fla4B, the transmission of parental heterozygosity was estimated in 2n eggs through randomly amplified polymorphic DNA (RAPD) analysis of 41 progeny from crosses of Fla4B to tetraploid V. corymbosum (2n = 4x = 48). The level of estimated heterozygosity transmitted ranged from 5.3-100%, with an overall mean heterozygosity of 72.7%, which suggests that first division restitution (FDR) type 2n gamete forming mechanisms are operating in this clone. However, there was evidence that 17% of the 2n eggs had less than 50% estimated heterozygosity, which supports second division restitution (SDR) mechanisms operating as well. Both SDR and FDR modes of 2n egg formation are also supported by a nonnormal, bimodal frequency distribution of estimated heterozygosity transmission. Recombination also appears to be prevalent. The predominance of 2n gametes genetically equivalent to FDR is generally considered desirable for breeding autotetraploid crops.

Original languageEnglish (US)
Pages (from-to)423-426
Number of pages4
JournalJournal of Heredity
Volume88
Issue number5
DOIs
StatePublished - 1997

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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