Evaluation of a hydrogel-fiber composite for ACL tissue engineering

Joseph W. Freeman, Mia D. Woods, Damond A. Cromer, Emmanuel C. Ekwueme, Tea Andric, Emmanuel A. Atiemo, Christian H. Bijoux, Cato T. Laurencin

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The anterior cruciate ligament (ACL) is necessary for normal knee stability and movement. Unfortunately the ACL is also the most frequently injured ligament of the knee with severe disruptions requiring surgical intervention. In response to this, tissue engineering has emerged as an option for ACL replacement and repair. In this study we present a novel hydrogel-fibrous scaffold as a potential option for ACL replacement. The scaffold was composed of PLLA fibers, in a previously evaluated braid-twist structure, combined with a polyethylene glycol diacrylate (PEGDA) hydrogelto improve viscoelastic properties. Both hydrogel concentration (10%, 15%, and 20%) and amount of hydrogel (soaking the fibrous scaffold in hydrogel solution or encasing the scaffold in a block of hydrogel) were evaluated. It was found that the braid-twist scaffold had a greater porosity and larger number of pores above 100 γm than braided scaffolds with the same braiding angle. After testing for their effects on swelling, fiber degradation, and protein release, as well as viscoelastic and tensile testing (when combined with fibrous scaffolds), it was found that the composite scaffold soaked in 10% hydrogel had the best chemical release and mechanical properties. The optimized structure behaved similarly to natural ligament in tension with the addition of the hydrogel decreasing the ultimate tensile stress (UTS), but the UTS was still comparable to natural ACL. In addition, cellular studies showed that the hydrogel-PLLA fiber composite supported fibroblast growth.

Original languageEnglish (US)
Pages (from-to)694-699
Number of pages6
JournalJournal of Biomechanics
Volume44
Issue number4
DOIs
StatePublished - Feb 24 2011

Fingerprint

Anterior Cruciate Ligament
Hydrogel
Ligaments
Tissue Engineering
Tissue engineering
Hydrogels
Scaffolds
Fibers
Composite materials
Tensile stress
Knee
Porosity
Tensile testing
Fibroblasts
Polyethylene glycols
Proteolysis
Swelling
Repair
Proteins
Degradation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

Keywords

  • Anterior cruciate ligament (ACL)
  • Ligament
  • Stress relaxation
  • Tensile
  • Tissue engineering

Cite this

Freeman, J. W., Woods, M. D., Cromer, D. A., Ekwueme, E. C., Andric, T., Atiemo, E. A., ... Laurencin, C. T. (2011). Evaluation of a hydrogel-fiber composite for ACL tissue engineering. Journal of Biomechanics, 44(4), 694-699. https://doi.org/10.1016/j.jbiomech.2010.10.043
Freeman, Joseph W. ; Woods, Mia D. ; Cromer, Damond A. ; Ekwueme, Emmanuel C. ; Andric, Tea ; Atiemo, Emmanuel A. ; Bijoux, Christian H. ; Laurencin, Cato T. / Evaluation of a hydrogel-fiber composite for ACL tissue engineering. In: Journal of Biomechanics. 2011 ; Vol. 44, No. 4. pp. 694-699.
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Freeman, JW, Woods, MD, Cromer, DA, Ekwueme, EC, Andric, T, Atiemo, EA, Bijoux, CH & Laurencin, CT 2011, 'Evaluation of a hydrogel-fiber composite for ACL tissue engineering', Journal of Biomechanics, vol. 44, no. 4, pp. 694-699. https://doi.org/10.1016/j.jbiomech.2010.10.043

Evaluation of a hydrogel-fiber composite for ACL tissue engineering. / Freeman, Joseph W.; Woods, Mia D.; Cromer, Damond A.; Ekwueme, Emmanuel C.; Andric, Tea; Atiemo, Emmanuel A.; Bijoux, Christian H.; Laurencin, Cato T.

In: Journal of Biomechanics, Vol. 44, No. 4, 24.02.2011, p. 694-699.

Research output: Contribution to journalArticle

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AU - Freeman, Joseph W.

AU - Woods, Mia D.

AU - Cromer, Damond A.

AU - Ekwueme, Emmanuel C.

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AU - Atiemo, Emmanuel A.

AU - Bijoux, Christian H.

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KW - Ligament

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