Evaluation of an atypical HIV type 1 antibody. Serologic pattern leading to detection of HIV type 2 infection in North America

J. M. Lombardo, C. R. Horsburgh, T. N. Denny, J. Michaels, C. Schable, M. F. Meyenhofer, K. Shriver, L. R. Sharer, C. Y. Ou, M. A. Rayfield, P. C. Kloser, J. M. Oleske, G. Schochetman, S. H. Weiss

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The variability and discordance of human immunodeficiency virus type 1 (HIV-1) antibody enzyme immunoassay determinations on serial specimens derived, to our knowledge, from the first documented case of HIV-2 infection in North America are described. The initial specimen was weakly reactive, but two subsequent serum specimens were both nonreactive by enzyme immunoassay. All specimens were indeterminate for HIV-1 antibody by HIV-1 Wstern blot analysis. Serum HIV-2 antibody was demonstrated by enzyme immunoassay using whole virus lysate, HIV-2-specific synthetic peptide assays, and HIV-2 Western blot analysis. Human immunodeficiency virus type 2 genomic sequences were demonstrated in peripheral blood mononuclear cells using gene amplification technology. Human immunodeficiency virus type 2, isolated from peripheral blood lymphocytes, had typical morphologic features of lentivirus by electron microscopy. Western blot analysis and other specific assays should be considered in individuals with clinical evidence suggesting HIV infection who are nonreactive for HIV-1 antibody by enzyme immunoassay or who have atypical reactivity patterns.

Original languageEnglish (US)
Pages (from-to)1245-1249
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume113
Issue number11
StatePublished - 1989

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

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