Evaluation of immunity to varicella zoster virus with a novel double antigen sandwich enzyme-linked immunosorbent assay

Jian Liu, Chunye Chen, Rui Zhu, Xiangzhong Ye, Jizong Jia, Lianwei Yang, Yongmei Wang, Wei Wang, Jianghui Ye, Yimin Li, Hua Zhu, Qinjian Zhao, Jun Zhang, Tong Cheng, Ningshao Xia

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Varicella is a highly contagious disease caused by primary infection of Varicella zoster virus (VZV). Varicella can be severe or even lethal in susceptible adults, immunocompromised patients and neonates. Determination of the status of immunity to VZV is recommended for these high-risk populations. Furthermore, measurement of population immunity to VZV can help in developing proper varicella vaccination programmes. VZV glycoprotein E (gE) is an antigen that has been demonstrated to be a highly accurate indicator of VZV-specific immunity. In this study, recombinant gE (rgE) was used to establish a double antigen sandwich enzyme-linked immunosorbent assay (ELISA). The established sandwich ELISA showed high specificity and sensitivity in the detection of human sera, and it could detect VZV-specific antibodies at a concentration of 11.25 m IU/mL with a detection linearity interval of 11.25 to 360 m IU/mL (R2 = 0.9985). The double gE antigen sandwich ELISA showed a sensitivity of 95.08 % and specificity of 100 % compared to the fluorescent-antibody-to-membrane-antigen (FAMA) test, and it showed a sensitivity of 100 % and a specificity of 94.74 % compared to a commercial neutralizing antibody detection kit. Thus, the established double antigen sandwich ELISA can be used as a sensitive and specific quantitative method to evaluate immunity to VZV.

Original languageEnglish (US)
Pages (from-to)9321-9329
Number of pages9
JournalApplied Microbiology and Biotechnology
Issue number21
StatePublished - Nov 1 2016

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology


  • Double antigen sandwich ELISA
  • Glycoprotein E
  • Serological evaluation
  • Varicella zoster virus


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