Evaluation of repetitive element polymerase chain reaction for surveillance of methicillin-resistant Staphylococcus aureus at a large academic medical center and community hospitals

Shu Hua Wang, Kurt B. Stevenson, Lisa Hines, José R. Mediavilla, Yosef Khan, Ruchi Soni, Wendy Dutch, Eric Brandt, Tammy Bannerman, Barry N. Kreiswirth, Preeti Pancholi

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Repetitive element polymerase chain reaction (rep-PCR) typing has been used for methicillin-resistant Staphylococcus aureus (MRSA) strain characterization. The goal of this study was to determine if a rapid commercial rep-PCR system, DiversiLab™ (DL; bioMérieux, Durham, NC, USA), could be used for MRSA surveillance at a large medical center and community hospitals. A total of 1286 MRSA isolates genotyped by the DL system were distributed into 84 distinct rep-PCR patterns: 737/1286 (57%) were clustered into 6 major rep-PCR patterns. A subset of 220 isolates was further typed by pulsed-field gel electrophoresis (PFGE), spa typing, and SCC. mec typing. The 220 isolates were distributed into 80 rep-PCR patterns, 94 PFGE pulsotypes, 27 spa, and 3 SCC. mec types. The DL rep-PCR system is sufficient for surveillance, but the DL system alone cannot be used to compare data to other institutions until a standardized nomenclature is established and the DL MRSA reference library is expanded.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume81
Issue number1
DOIs
StatePublished - Jan 1 2015

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Keywords

  • MRSA
  • Methicillin-resistant staphylococcus aureus
  • Molecular genotyping
  • PFGE
  • Rep-PCR
  • SCCmec
  • Spa

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