Evaluation of the precision of intradermal injection of control substances for intradermal testing in clinically normal horses

David M. Wong, Virginia A. Buechner-Maxwell, Thomas O. Manning, Daniel Ward

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective - To evaluate the precision of intradermal testing (IDT) in horses. Animals - 12 healthy adult horses. Procedure - IDT was performed on the neck of each horse by use of 2 positive control substances (histamine and phytohemagglutinin [PHA]) and a negative control substance. An equal volume (0.1 mL) for each injection was prepared to yield a total of 20 syringes ([4 concentrations of each positive control substance plus 1 negative control substance] times 2 positive control substances times 2 duplicative tests) for each side of the neck. Both sides of the neck were used for IDT; therefore, 40 syringes were prepared for each horse. Hair was clipped on both sides of the neck, and ID injections were performed. Diameter of the skin wheals was recorded 0.5, 4, and 24 hours after ID injection. Results - Intra- and interhorse skin reactions to ID injection of histamine and PHA resulted in wheals of uniform size at 0.5 and 4 hours, respectively. Significant intra- and interhorse variation was detected in wheals caused by PHA at 24 hours. Conclusions and clinical relevance - ID injection of histamine and PHA caused repeatable and precise results at 0.5 and 4 hours, respectively. Concentrations of 0.005 mg of histamine/mL and 0.1 mg of PHA/mL are recommended for use as positive control substances for IDT in horses. This information suggests that consistent wheal size is evident for ID injection of control substances, and variation in wheals in response to ID injection of test antigens results from a horse's immune response to specific antigens.

Original languageEnglish (US)
Pages (from-to)1341-1347
Number of pages7
JournalAmerican journal of veterinary research
Volume66
Issue number8
DOIs
StatePublished - Aug 1 2005
Externally publishedYes

Fingerprint

Intradermal Injections
Horses
phytohemagglutinin
Phytohemagglutinins
injection
horses
Injections
histamine
Histamine
neck
Neck
testing
syringes
Syringes
skin (animal)
antigens
Antigens
Skin
Hair
hairs

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

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title = "Evaluation of the precision of intradermal injection of control substances for intradermal testing in clinically normal horses",
abstract = "Objective - To evaluate the precision of intradermal testing (IDT) in horses. Animals - 12 healthy adult horses. Procedure - IDT was performed on the neck of each horse by use of 2 positive control substances (histamine and phytohemagglutinin [PHA]) and a negative control substance. An equal volume (0.1 mL) for each injection was prepared to yield a total of 20 syringes ([4 concentrations of each positive control substance plus 1 negative control substance] times 2 positive control substances times 2 duplicative tests) for each side of the neck. Both sides of the neck were used for IDT; therefore, 40 syringes were prepared for each horse. Hair was clipped on both sides of the neck, and ID injections were performed. Diameter of the skin wheals was recorded 0.5, 4, and 24 hours after ID injection. Results - Intra- and interhorse skin reactions to ID injection of histamine and PHA resulted in wheals of uniform size at 0.5 and 4 hours, respectively. Significant intra- and interhorse variation was detected in wheals caused by PHA at 24 hours. Conclusions and clinical relevance - ID injection of histamine and PHA caused repeatable and precise results at 0.5 and 4 hours, respectively. Concentrations of 0.005 mg of histamine/mL and 0.1 mg of PHA/mL are recommended for use as positive control substances for IDT in horses. This information suggests that consistent wheal size is evident for ID injection of control substances, and variation in wheals in response to ID injection of test antigens results from a horse's immune response to specific antigens.",
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Evaluation of the precision of intradermal injection of control substances for intradermal testing in clinically normal horses. / Wong, David M.; Buechner-Maxwell, Virginia A.; Manning, Thomas O.; Ward, Daniel.

In: American journal of veterinary research, Vol. 66, No. 8, 01.08.2005, p. 1341-1347.

Research output: Contribution to journalArticle

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