Evidence for the presence of N-acetylaspartylglutamate in cultured oligodendrocytes and LPS activated microglia

L. Passani, S. Elkabes, J. T. Coyle

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The levels of N-acetylaspartylglutamate (NAAG) were determined by HPLC in untreated or lipopolysaccharide (LPS) activated pure astrocyte, oligodendrocyte, and microglial cultures derived from developing rat brain. Oligodendrocyte cultures expressed 1.52 ± 0.12 nmol/μg protein of NAAG, whereas astrocyte cultures (0.04 ± 0.08 nmol/μg protein) and untreated microglial cultures (0.05 ± 0.09 nmol/μg protein) contained only trace mounts of the dipeptide. After stimulation of microglial cultures for 24 h with LPS, NAAG levels increased significantly to 0.37 ± 0.12 SD nmol/μg protein. NAAG levels in astrocyte and oligodendrocyte cultures remained unchanged after LPS treatment. The findings indicate that NAAG is localized to specific glial cell types. Further our results suggest that NAAG biosynthesis is induced in microglia, activated by specific stimuli.

Original languageEnglish (US)
Pages (from-to)143-145
Number of pages3
JournalBrain research
Volume794
Issue number1
DOIs
StatePublished - May 25 1998

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Keywords

  • Alzheimer's disease
  • Excitatory amino acid
  • Huntington's disease
  • Microgilia
  • N-acetylaspartylglutamate
  • Oligodendrocyte
  • Peptide

Fingerprint Dive into the research topics of 'Evidence for the presence of N-acetylaspartylglutamate in cultured oligodendrocytes and LPS activated microglia'. Together they form a unique fingerprint.

Cite this