Evidence for three additional P2X2 purinoceptor isoforms produced by alternative splicing in the adult rat vestibular end-organs

Marta Troyanovskaya, Phillip A. Wackym

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22 Scopus citations

Abstract

P2X2 receptors are ligand-gated ion channels that are activated by extracellular ATP. To characterize the expression of P2X2 purinoceptor in the adult rat vestibular periphery, reverse transcription-polymerase chain reaction (RT-PCR) was used. No transcript for P2X2 receptor was found in the vestibular primary afferent neurons (Scarpa's ganglia); however, partial cDNAs encoding four splice variants of the P2X2 receptor were isolated from vestibular end-organs. In all four cDNAs, the deletions were of different lengths but started at the same position on the P2X2 gene (Val-370 codon) located toward the intracellular carboxyl terminus. One of these receptor isoforms was identical in sequence to the recently published P2X(2(b)) receptor (Simon et al., 1997, Mol. Pharmacol. 52, 237-248) (also known as P2X2-2, in the nomenclature of Brandle et al, 1997, FEBS Lett. 404, 294- 298). The remaining three novel splice variants of the P2X2 receptor were designated P2X(2(e)), P2X(2(f)) and P2X(2(g)) (GenBank accession numbers AF028603, AF028604 and AF028605, respectively). The functional significance of these three splice variants remains to be determined. Pituitary and cerebellum were used as survey tissues and only the P2X(2(b)) receptor cDNA was found.

Original languageEnglish (US)
Pages (from-to)201-209
Number of pages9
JournalHearing Research
Volume126
Issue number1-2
DOIs
StatePublished - Dec 1998

All Science Journal Classification (ASJC) codes

  • Sensory Systems

Keywords

  • Alternative splicing
  • Extracellular ATP
  • Ligand-gated channel
  • P2 purinoceptor
  • Vestibular periphery

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