Experimental colitis impairs linear bone growth independent of nutritional factors

Soula G. Koniaris, Stanley E. Fisher, Clinton T. Rubin, Anupama Chawla

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32 Scopus citations

Abstract

Background: Poor linear growth frequently complicates chronic inflammatory bowel disease in children. Circulating inflammatory mediators may play a role in this growth delay. We evaluated the effect of experimental colitis on bone growth in a nutritionally controlled rat model. Methods: Experimental colitis was induced in male Sprague-Dawley rats (125-150 g) by enema with trinitrobenzene sulfonic acid in 50% ethanol on day 1 and 11 of a 14-day protocol. Control animals were pair-fed and all animals received a liquid rat diet (1 kcal/ml). Twenty-four-hour urine, collected on days 2 and 12 and serum samples, collected at death, were analyzed for calcium, zinc, and magnesium. Serum samples from a separate set of animals were studied for serial interleukin-6 levels. Right proximal tibias were processed for growth- plate histomorphometry, in which linear growth is proportional to the heights of the proliferative zone, and terminal hypertrophic chondrocyte, but inversely proportional to the height of the resting zone. Results: Histology confirmed active inflammation in the animals given trinitrobenzene sulfonic acid. Weight gain and both urinary excretion and serum levels of zinc, calcium, and magnesium did not differ between treatment and nontreatment groups. Histologically, there was impaired linear bone growth. The resting zone was greater in the colitis group (94.5 ± 32.6 μm versus 3.9 ± 5.4 μm; p < 0.05); the proliferative zone was smaller in the colitis group (123.7 ± 18.2 μm versus 78.9 ± 11.2; p < 0.05 μm); the terminal hypertrophic chondrocyte was reduced in the colitis group (19.5 ± 1.4 μm versus 28.8 ± 3.6 μm; p < 0.05). At 6 and 24 hours after induction, the level of interleukin-6 was elevated in the colitis group. Conclusions: Experimental colitis results in a decreased linear bone growth, independent of nutritional intake. Circulating cytokines derived from intestinal inflammation may contribute to the suppression of bone growth.

Original languageEnglish (US)
Pages (from-to)137-141
Number of pages5
JournalJournal of pediatric gastroenterology and nutrition
Volume25
Issue number2
DOIs
Publication statusPublished - Aug 1 1997
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Gastroenterology

Keywords

  • Bone growth
  • Colitis
  • Interleukin-6
  • Rats
  • Trinitrobenzene sulfonic acid

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