Experimental demonstration and numerical simulation of organic-aqueous liquid extraction enhanced by droplet formation in a microfluidic channel

Xiaole Mao, Sung Yang, Jeffrey D. Zahn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Organic-aqueous (phenol) liquid extraction is one of the commonly used DNA purification methods. Effective mass transfer of biological material between the discrete fluid phases is key to achieving efficient extraction when designing microfluidic devices based on this technique. In the microscale regime, mass transfer is often diffusion limited. However, mass transfer can be enhanced through the formation of discrete droplets within a microchannel, which leads to a recirculation flow pattern within the droplet. This recirculation increases the mass transfer rate of material to the organic-aqueous interface. In this study, an experimental and computational examination of sample extraction between the organic and aqueous phases through droplet formation is presented. The experiment is conducted within a converging dual inlet microfluidic channel fabricated in PDMS. By controlling the capillary number of the flow, different flow patterns are created in the channel. The flow patterns are examined using a computational fluid dynamics (CFD) simulation. The CFD model successfully simulates the flow behavior under a variety of flow conditions and provides a closer examination of the internal recirculation pattern within the droplet. The experimental sample extraction utilizes a fluorescent dye localization technique and shows that the droplet flow offers a significant improvement in the speed of sample extraction over diffusional mixing. A preliminary test demonstrates the feasibility of using the droplet formation for fast extraction with biological samples.

Original languageEnglish (US)
Title of host publicationProceedings of 2006 ASME International Mechanical Engineering Congress and Exposition, IMECE2006 - Fluids Engineering Division
PublisherAmerican Society of Mechanical Engineers (ASME)
ISBN (Print)0791837904, 9780791837900
DOIs
StatePublished - 2006
Externally publishedYes
Event2006 ASME International Mechanical Engineering Congress and Exposition, IMECE2006 - Chicago, IL, United States
Duration: Nov 5 2006Nov 10 2006

Publication series

NameAmerican Society of Mechanical Engineers, Fluids Engineering Division (Publication) FED
ISSN (Print)0888-8116

Other

Other2006 ASME International Mechanical Engineering Congress and Exposition, IMECE2006
CountryUnited States
CityChicago, IL
Period11/5/0611/10/06

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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