Uniform practices and quality control methods are needed to detect and quantify airborne viruses across sampling and analysis platforms. We compared detection of airborne SARSCoV-2 RNA in residences of individuals with COVID-19 using two commonly used criteria: environmental (at least one SARS-CoV-2-specific gene and internal control amplified by PCR with Ct ≤ 40) and clinical (at least two SARS-CoV-2-specific genes and internal control amplified with Ct ≤ 37). 24-hr total aerosol samples were collected in a self-isolation room and an additional room without manipulating subjects' behavior/activities. Under the environmental criterion, 7/16 samples in primary rooms and 7/15 samples in secondary rooms were positive. Comparable but lower positive sample proportions were observed using the more rigorous clinical criterion: 6/16 primary rooms and 5/15 secondary rooms. A consensus SARS-CoV-2 environmental sampling and analysis framework is needed for comparisons between studies.
|Original language||English (US)|
|State||Published - 2022|
|Event||17th International Conference on Indoor Air Quality and Climate, INDOOR AIR 2022 - Kuopio, Finland|
Duration: Jun 12 2022 → Jun 16 2022
|Conference||17th International Conference on Indoor Air Quality and Climate, INDOOR AIR 2022|
|Period||6/12/22 → 6/16/22|
All Science Journal Classification (ASJC) codes