Expression and secretion of chicken apolipoprotein AI in transfected COS cells

Joseph L. Dixon, Renata Battini, Stefano Ferrari, Colvin M. Redman, Debendranath Banerjee

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9 Scopus citations


A full-length chicken apolipoprotein A-I (apoAI) cDNA has been cloned into an expression vector, pRSVapoAI. This plasmid was transfected into a monkey kidney (COS-1) cell line in order to study apolipoprotein-lipid assembly. Chicken apoAI is the major apolipoprotein of chicken high-density lipoprotein-lipid assembly. apolipoprotein content than the HDL of human plasma. The transient transfected COS-1 cells synthesized and secreted authentic plasma apoAI. Under serum-free medium conditions, COS cells secreted only proapoAI. A small portion (15%) of the secreted apoAI floated at a density 1.07-1.20 g/ml. Upon incubation with fetal bovine serum at 10°C, a majority of the apoAI was recovered in the HDL density (1.06-1.20 g/ml) region. Secreted apoAI was labeled when transfected COS cells were incubated with [U-14C]palmitate, but the incorporation of radioactivity was not the result of fatty acid acylation through ester bond formation. These results indicate that heterologous COS-1 cells are capable of synthesizing and secreting apoAI, and that intracellular association of apoAI with lipids is not necessary for secretion.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalBBA - Gene Structure and Expression
Issue number1
StatePublished - Sep 21 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics


  • (Chicken)
  • Apolipoprotein
  • HDL
  • Lipoprotein assembly
  • Secretion
  • Transfection


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