Expression of activated checkpoint kinase 2 and histone 2AX in exfoliative oral cells after exposure to ionizing radiation

Angela J. Yoon, Jing Shen, Hui Chen Wu, Christos Angelopoulos, Steven R. Singer, Rongzhen Chen, Regina M. Santella

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

γ-H2AX (activated histone 2AX) and pChk2 (activated checkpoint kinase 2), which are DNA damage response molecules, are produced in irradiated cells and may be signature molecules of radiation exposure. We investigated their use as potential biomarkers to identify individuals exposed to ionizing radiation. We collected exfoliated oral epithelial cell samples from 100 healthy individuals undergoing routine dental radiographic examination (2.34 cGy) both before and after the radiograph using a non-invasive technique. The expression levels of pChk2 and γ-H2AX in oral cells were assessed by immunohistochemical assay. Both biomarkers showed statistically significant increases in levels of expression after the radiation exposure (P < 0.001). This suggests that pChk2 and γ-H2AX may serve as sensitive indicators of low-dose radiation exposure.

Original languageEnglish (US)
Pages (from-to)771-775
Number of pages5
JournalRadiation Research
Volume171
Issue number6
DOIs
StatePublished - Jun 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Radiation
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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