Expression of monocyte-specific oncogenes c-fos and c-fms in HL60 cells treated with vitamin D3 analogs correlates with inhibition of DNA synthesis and reduced calmodulin concentration

Z. S. Brelvi, S. Christakos, G. P. Studzinski

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Monocytic differentiation of cultured leukemic cells HL60 can be induced by a variety of compounds including analogs of vitamin D3. We studied the expression of oncogenes c-fos, c-fms, c-fes, c-myb, c-myc, and c-Ha-ras during this process, and attempted to relate these and a conventional marker of monocytic differentiation, the nonspecific esterase activity to changes in cytosol levels of the Ca2+-binding proteins. The analogs of vitamin D3, 1α,25-dihydroxycholecalciferol, 1α,25-dihydroxy-26,27-hexafluorocholecalciferol, and 1α,25-dihydroxy-24R-fluorocholecalciferol, reduced the total calcium binding activity and the cellular levels of calmodulin, but calbindin(28k) could not be detected in HL 60 cells. A correlation was evident between the potency of these compounds as inducers of differentiation demonstrated by nonspecific esterase activity, the degree of reduction in calmodulin concentration, the rate of the inhibition of DNA synthesis and of expression of c-myc and c-myb genes, and the induction of the expression of oncogenes c-fos and c-fms. These experiments indicate that the events which underlie monocytic differentiation of HL 60 cells can be observed to occur in discrete steps which include an inhibition of DNA synthesis, a reduction of cellular levels of calmodulin, and altered expression of several oncogenes.

Original languageEnglish (US)
Pages (from-to)269-275
Number of pages7
JournalLaboratory Investigation
Volume55
Issue number3
StatePublished - Jan 1 1986

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Expression of monocyte-specific oncogenes c-fos and c-fms in HL60 cells treated with vitamin D<sub>3</sub> analogs correlates with inhibition of DNA synthesis and reduced calmodulin concentration'. Together they form a unique fingerprint.

Cite this