Expression turnover profiling to monitor the antifungal activities of amphotericin B, voriconazole, and micafungin against Aspergillus fumigatus

Yanan Zhao; Padmaja Paderu; Steven Park; Aleksandra Dukhan; Meredith Senter; David S. Perlin

doi:10.1128/AAC.06163-11

Expression turnover profiling to monitor the antifungal activities of amphotericin B, voriconazole, and micafungin against Aspergillus fumigatus

Yanan Zhao, Padmaja Paderu, Steven Park, Aleksandra Dukhan, Meredith Senter, David S. Perlin

New Jersey Medical School (NJMS), Public Health Research Institute Center

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Eight highly expressed candidate genes were selected for mRNA profiling to monitor the transcriptome kinetics of Aspergillus fumigatus strains exposed to antifungal drugs as potential biomarkers of live cells to assess treatment efficacy. Mycelia were treated with fungicidal drugs amphotericin B and voriconazole, as well as the fungistatic drug micafungin. Transcription was monitored at 0, 4, 8, and 24 h posttreatment. The expression turnover profile provides a possible tool to assess antifungal therapy effects.

Original language	English (US)
Pages (from-to)	2770-2772
Number of pages	3
Journal	Antimicrobial agents and chemotherapy
Volume	56
Issue number	5
DOIs	https://doi.org/10.1128/AAC.06163-11
State	Published - May 2012

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmacology (medical)
Infectious Diseases

Access to Document

10.1128/AAC.06163-11

Cite this

@article{b8f36899b2b74344a54d26dbeb60a157,

title = "Expression turnover profiling to monitor the antifungal activities of amphotericin B, voriconazole, and micafungin against Aspergillus fumigatus",

abstract = "Eight highly expressed candidate genes were selected for mRNA profiling to monitor the transcriptome kinetics of Aspergillus fumigatus strains exposed to antifungal drugs as potential biomarkers of live cells to assess treatment efficacy. Mycelia were treated with fungicidal drugs amphotericin B and voriconazole, as well as the fungistatic drug micafungin. Transcription was monitored at 0, 4, 8, and 24 h posttreatment. The expression turnover profile provides a possible tool to assess antifungal therapy effects.",

author = "Yanan Zhao and Padmaja Paderu and Steven Park and Aleksandra Dukhan and Meredith Senter and Perlin, {David S.}",

year = "2012",

month = may,

doi = "10.1128/AAC.06163-11",

language = "English (US)",

volume = "56",

pages = "2770--2772",

journal = "Antimicrobial agents and chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "5",

}

TY - JOUR

T1 - Expression turnover profiling to monitor the antifungal activities of amphotericin B, voriconazole, and micafungin against Aspergillus fumigatus

AU - Zhao, Yanan

AU - Paderu, Padmaja

AU - Park, Steven

AU - Dukhan, Aleksandra

AU - Senter, Meredith

AU - Perlin, David S.

PY - 2012/5

Y1 - 2012/5

N2 - Eight highly expressed candidate genes were selected for mRNA profiling to monitor the transcriptome kinetics of Aspergillus fumigatus strains exposed to antifungal drugs as potential biomarkers of live cells to assess treatment efficacy. Mycelia were treated with fungicidal drugs amphotericin B and voriconazole, as well as the fungistatic drug micafungin. Transcription was monitored at 0, 4, 8, and 24 h posttreatment. The expression turnover profile provides a possible tool to assess antifungal therapy effects.

AB - Eight highly expressed candidate genes were selected for mRNA profiling to monitor the transcriptome kinetics of Aspergillus fumigatus strains exposed to antifungal drugs as potential biomarkers of live cells to assess treatment efficacy. Mycelia were treated with fungicidal drugs amphotericin B and voriconazole, as well as the fungistatic drug micafungin. Transcription was monitored at 0, 4, 8, and 24 h posttreatment. The expression turnover profile provides a possible tool to assess antifungal therapy effects.

UR - http://www.scopus.com/inward/record.url?scp=84860176891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860176891&partnerID=8YFLogxK

U2 - 10.1128/AAC.06163-11

DO - 10.1128/AAC.06163-11

M3 - Article

C2 - 22314535

AN - SCOPUS:84860176891

SN - 0066-4804

VL - 56

SP - 2770

EP - 2772

JO - Antimicrobial agents and chemotherapy

JF - Antimicrobial agents and chemotherapy

IS - 5

ER -

Expression turnover profiling to monitor the antifungal activities of amphotericin B, voriconazole, and micafungin against Aspergillus fumigatus

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this