Extracellular electron shuttling mediated by soluble c-type cytochromes produced by Shewanella oneidensis MR-1

Tongxu Liu, Xiaobo Luo, Yundang Wu, John R. Reinfelder, Xiu Yuan, Xiaomin Li, Dandan Chen, Fangbai Li

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


How metal-reducing bacteria transfer electrons during dissimilatory energy generation under electron acceptor-limited conditions is poorly understood. Here, we incubated the iron and manganese-reducing bacterium Shewanella oneidensis MR-1 without electron acceptors. Removal of soluble extracellular organic compounds (EOCs) dramatically retarded transfer of electrons to an experimental electron acceptor, Cr(VI), by MR-1. However, the return of either high MW (>3000 Da) or low MW (<3000 Da) soluble EOCs produced by MR-1 to washed cells restored Cr(VI) reduction though Cr(VI) reduction was fastest when both size fractions were added together. Spectral and electrochemical characterization of EOCs indicated the presence of flavins and c-type cytochromes (c-Cyts). A model of the kinetics of individual elementary reactions between cells, flavins, released c-Cyts, and Cr(VI), including the direct reduction of flavins, released c-Cyts, and Cr(VI) by cells and the indirect reduction of Cr(VI) by reduced forms of flavins and released c-Cyts, was developed. Model results suggest that released c-Cyts could act as electron mediators to accelerate electron transfer from cells to Cr(VI), and the relative contribution of this pathway was higher than that mediated by flavins. Hence, extracellular c-Cyts produced by MR-1 likely play a role in extracellular electron transfer under electron acceptor-limited conditions. These findings provide new insights into extracellular electron shuttling and the metabolic strategy of metal-reducing bacteria under electron acceptor-limited conditions.

Original languageEnglish (US)
Pages (from-to)10577-10587
Number of pages11
JournalEnvironmental Science and Technology
Issue number17
StatePublished - Sep 1 2020

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry


Dive into the research topics of 'Extracellular electron shuttling mediated by soluble c-type cytochromes produced by Shewanella oneidensis MR-1'. Together they form a unique fingerprint.

Cite this