Extraction and quantification of benzo[a]pyrene in soil by reversed phase thin layer chromatography

D. R. Nemergut, R. M. Johnson, K. G. Wunch, J. W. Bennett

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


As research on the biodegradation of benzo[a]pyrene (B[a]P) shifts from laboratory to field based studies, a need has arisen for reproducible, economical, and accurate methods for extraction and quantification of B[a]P. This study describes a method for the analysis of B[a]P using batch extraction techniques with quantification by reversed phase high performance thin layer chromatography (HPTLC) and densitometry. A loam soil was fortified with B[a]P at a level of 100 mg B[a]P kg-1 soil. Several solvents were evaluated as potential soil extraction systems including: acetone, acetonitrile, ethyl acetate, methanol, and methylene chloride:acetone (1:1). Reverse phase thin layer chromatography of B[a]P was performed on hydrocarbon impregnated, C18 reverse phase plates using methanol:acetonitrile (1:1) as the developing solvent. The Rf for B[a]P in this system was found to be 0.52. Recovery of B[a]P from soil ranged from 11% with methanol to 84% with ethyl acetate.

Original languageEnglish (US)
Pages (from-to)579-586
Number of pages8
JournalJournal of Liquid Chromatography and Related Technologies
Issue number4
StatePublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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