Factors affecting the efficiency of protein synthesis in Escherichia coli. Production of a polypeptide of more than 6000 amino acid residues

K. Tsung, S. Inouye, M. Inouye

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29 Scopus citations

Abstract

Factors affecting the efficiency of protein synthesis were analyzed in Escherichia coli. For this purpose the lacZ gene was fused to produce polypeptides from a dimer (molecular weight 229,957) to a hexamer (molecular weight 684,924) of β-galactosidase. From pulse-chase experiments it was found that only 45% of the ribosomes which reached to the end of the first monomer were able to complete the second monomer unit. Similarly, for every addition of a monomer unit to synthesize the multimers from the trimer to the hexamer approximately half of the ribosomes failed to complete the synthesis of the added unit. Furthermore, the stability of the polypeptides decreased as their sizes increased. As a result, the overall efficiency of the production of the β-galactosidase polymers dropped by a factor of approximately 3 on a weight basis for each addition of a monomer unit.

Original languageEnglish (US)
Pages (from-to)4428-4433
Number of pages6
JournalJournal of Biological Chemistry
Volume264
Issue number8
StatePublished - 1989

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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