The initial attachment of transforming DNA to the competent Bacillus subtilis cell surface is sensitive to shear. This is interpreted as indicating that the attachment is terminal. Sensitivity to shear disappears as the donor molecules are converted to DSF. Using isolation procedures involving minimal shear forces, DNA-cell complexes may be isolated, permitting recovery of intact donor DNA. This donor component decreases in amount and in average length as DSF accumulates in the cell, indicating that donor DNA is converted to DSF molecules starting from one end. Kinetic evidence is presented showing that DSF must be on the main pathway leading to recombinant formation. Based on these and previous observations a working model is presented, describing the transformation process in B. subtilis.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology