Fatty acid amide of glycerylphosphorylethanolamine-A lytic lipid from hog stomach

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

1. 1. A lipid composed of glycerol, phosphorus, ethanolamine and fatty acids has been isolated from hog stomach lipids by column fractionations on DEAE-cellulose and silicic acid followed by purification on thin-layer plates. 2. 2. The purified lipid exhibited strong lytic activity toward red blood and cultured rabbit kidney cells. 3. 3. Infrared spectra of the lytic lipid were characterized primarily by the strong absorption typical of secondary amide in addition to absorption indicative of phosphate ester groups and by the absence of absorption from C = O of esters. 4. 4. Treatment of the lytic lipid with periodate resulted in destruction of glycerol indicating the presence of free vicinal hydroxyl groups on the glycerol part of the lipid molecule. 5. 5. Glycerol, glycerylphosphate, fatty acid amide of ethanolamine and fatty acid amide of phosphorylethanolamine were identified from the products of partial acid hydrolysis of the lytic lipid. 6. 6. Glycerylphosphate and fatty acid amide of ethanolamine have been identified as products resulting from the treatment of the lytic lipid with phospholipase D. Treatment of the lipid with phospholipase C resulted only in a partial release of glycerol. 7. 7. Hexadecenoate, tetradecanoate and hexadecanoate were the principal fatty acids: fatty acids longer than C18 were absent. 8. 8. The isolated lipid appears to be a fatty acid amide of glycerylphosphorylethanolamine.

Original languageEnglish (US)
Pages (from-to)35-47
Number of pages13
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume316
Issue number1
DOIs
StatePublished - Jul 19 1973
Externally publishedYes

Fingerprint

Amides
Stomach
Fatty Acids
Lipids
Glycerol
Ethanolamine
Myristates
Esters
Phosphorus Acids
glycerophosphoethanolamine
Silicic Acid
DEAE-Cellulose
Phospholipase D
Palmitates
Distillation columns
Type C Phospholipases
Fractionation
Hydroxyl Radical
Phosphorus
Purification

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Endocrinology

Cite this

@article{f3635328e043456a82e6470f3efb8958,
title = "Fatty acid amide of glycerylphosphorylethanolamine-A lytic lipid from hog stomach",
abstract = "1. 1. A lipid composed of glycerol, phosphorus, ethanolamine and fatty acids has been isolated from hog stomach lipids by column fractionations on DEAE-cellulose and silicic acid followed by purification on thin-layer plates. 2. 2. The purified lipid exhibited strong lytic activity toward red blood and cultured rabbit kidney cells. 3. 3. Infrared spectra of the lytic lipid were characterized primarily by the strong absorption typical of secondary amide in addition to absorption indicative of phosphate ester groups and by the absence of absorption from C = O of esters. 4. 4. Treatment of the lytic lipid with periodate resulted in destruction of glycerol indicating the presence of free vicinal hydroxyl groups on the glycerol part of the lipid molecule. 5. 5. Glycerol, glycerylphosphate, fatty acid amide of ethanolamine and fatty acid amide of phosphorylethanolamine were identified from the products of partial acid hydrolysis of the lytic lipid. 6. 6. Glycerylphosphate and fatty acid amide of ethanolamine have been identified as products resulting from the treatment of the lytic lipid with phospholipase D. Treatment of the lipid with phospholipase C resulted only in a partial release of glycerol. 7. 7. Hexadecenoate, tetradecanoate and hexadecanoate were the principal fatty acids: fatty acids longer than C18 were absent. 8. 8. The isolated lipid appears to be a fatty acid amide of glycerylphosphorylethanolamine.",
author = "Slomiany, {Bronislaw L.} and Amalia Slomiany and Horowitz, {Martin I.}",
year = "1973",
month = "7",
day = "19",
doi = "10.1016/0005-2760(73)90164-1",
language = "English (US)",
volume = "316",
pages = "35--47",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Fatty acid amide of glycerylphosphorylethanolamine-A lytic lipid from hog stomach

AU - Slomiany, Bronislaw L.

AU - Slomiany, Amalia

AU - Horowitz, Martin I.

PY - 1973/7/19

Y1 - 1973/7/19

N2 - 1. 1. A lipid composed of glycerol, phosphorus, ethanolamine and fatty acids has been isolated from hog stomach lipids by column fractionations on DEAE-cellulose and silicic acid followed by purification on thin-layer plates. 2. 2. The purified lipid exhibited strong lytic activity toward red blood and cultured rabbit kidney cells. 3. 3. Infrared spectra of the lytic lipid were characterized primarily by the strong absorption typical of secondary amide in addition to absorption indicative of phosphate ester groups and by the absence of absorption from C = O of esters. 4. 4. Treatment of the lytic lipid with periodate resulted in destruction of glycerol indicating the presence of free vicinal hydroxyl groups on the glycerol part of the lipid molecule. 5. 5. Glycerol, glycerylphosphate, fatty acid amide of ethanolamine and fatty acid amide of phosphorylethanolamine were identified from the products of partial acid hydrolysis of the lytic lipid. 6. 6. Glycerylphosphate and fatty acid amide of ethanolamine have been identified as products resulting from the treatment of the lytic lipid with phospholipase D. Treatment of the lipid with phospholipase C resulted only in a partial release of glycerol. 7. 7. Hexadecenoate, tetradecanoate and hexadecanoate were the principal fatty acids: fatty acids longer than C18 were absent. 8. 8. The isolated lipid appears to be a fatty acid amide of glycerylphosphorylethanolamine.

AB - 1. 1. A lipid composed of glycerol, phosphorus, ethanolamine and fatty acids has been isolated from hog stomach lipids by column fractionations on DEAE-cellulose and silicic acid followed by purification on thin-layer plates. 2. 2. The purified lipid exhibited strong lytic activity toward red blood and cultured rabbit kidney cells. 3. 3. Infrared spectra of the lytic lipid were characterized primarily by the strong absorption typical of secondary amide in addition to absorption indicative of phosphate ester groups and by the absence of absorption from C = O of esters. 4. 4. Treatment of the lytic lipid with periodate resulted in destruction of glycerol indicating the presence of free vicinal hydroxyl groups on the glycerol part of the lipid molecule. 5. 5. Glycerol, glycerylphosphate, fatty acid amide of ethanolamine and fatty acid amide of phosphorylethanolamine were identified from the products of partial acid hydrolysis of the lytic lipid. 6. 6. Glycerylphosphate and fatty acid amide of ethanolamine have been identified as products resulting from the treatment of the lytic lipid with phospholipase D. Treatment of the lipid with phospholipase C resulted only in a partial release of glycerol. 7. 7. Hexadecenoate, tetradecanoate and hexadecanoate were the principal fatty acids: fatty acids longer than C18 were absent. 8. 8. The isolated lipid appears to be a fatty acid amide of glycerylphosphorylethanolamine.

UR - http://www.scopus.com/inward/record.url?scp=0015694361&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015694361&partnerID=8YFLogxK

U2 - 10.1016/0005-2760(73)90164-1

DO - 10.1016/0005-2760(73)90164-1

M3 - Article

C2 - 4352923

AN - SCOPUS:0015694361

VL - 316

SP - 35

EP - 47

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 1

ER -