1. 1. A lipid composed of glycerol, phosphorus, ethanolamine and fatty acids has been isolated from hog stomach lipids by column fractionations on DEAE-cellulose and silicic acid followed by purification on thin-layer plates. 2. 2. The purified lipid exhibited strong lytic activity toward red blood and cultured rabbit kidney cells. 3. 3. Infrared spectra of the lytic lipid were characterized primarily by the strong absorption typical of secondary amide in addition to absorption indicative of phosphate ester groups and by the absence of absorption from C = O of esters. 4. 4. Treatment of the lytic lipid with periodate resulted in destruction of glycerol indicating the presence of free vicinal hydroxyl groups on the glycerol part of the lipid molecule. 5. 5. Glycerol, glycerylphosphate, fatty acid amide of ethanolamine and fatty acid amide of phosphorylethanolamine were identified from the products of partial acid hydrolysis of the lytic lipid. 6. 6. Glycerylphosphate and fatty acid amide of ethanolamine have been identified as products resulting from the treatment of the lytic lipid with phospholipase D. Treatment of the lipid with phospholipase C resulted only in a partial release of glycerol. 7. 7. Hexadecenoate, tetradecanoate and hexadecanoate were the principal fatty acids: fatty acids longer than C18 were absent. 8. 8. The isolated lipid appears to be a fatty acid amide of glycerylphosphorylethanolamine.
|Original language||English (US)|
|Number of pages||13|
|Journal||Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism|
|State||Published - Jul 19 1973|
All Science Journal Classification (ASJC) codes