Fatty acid-induced Ca2+-dependent uncoupling and activation of external pathway of NADH oxidation are coupled to cyclosporin A-sensitive mitochondrial permeability transition

A. A. Starkov; O. V. Markova; E. N. Mokhova; E. Arrigoni-Martelli; V. A. Bobyleva

Fatty acid-induced Ca²⁺-dependent uncoupling and activation of external pathway of NADH oxidation are coupled to cyclosporin A-sensitive mitochondrial permeability transition

A. A. Starkov, O. V. Markova, E. N. Mokhova, E. Arrigoni-Martelli, V. A. Bobyleva

Research output: Contribution to journal › Article › peer-review

Abstract

Permeabilization of inner mitochondrial membrane by palmitic acid in the presence of Ca²⁺ (cyclosporin A-sensitive stimulation of respiration, decrease of Δψ and high amplitude swelling) is accompanied by activation of the external pathway of NADH oxidation in liver mitochondria. The 'pore'-sealing agents (cyclosporin A, Mg²⁺ with ADP, and L-carnitine with ATP) are equally effective in preventing the induction of external pathway of NADH oxidation by Ca²⁺ with palmitate. However, activities of these agents are different in respect to recoupling of permeabilized mitochondria. Participation of cyclosporin A-sensitive 'pore' in the fatty acid- and Ca²⁺-dependent induction of external pathway of NADH oxidation and in Ca²⁺-dependent uncoupling is discussed.

Original language	English (US)
Pages (from-to)	1147-1155
Number of pages	9
Journal	Biochemistry and Molecular Biology International
Volume	32
Issue number	6
State	Published - 1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Genetics

Cite this

@article{e8ae4f50887d435aa28ff71d7b6aba47,

title = "Fatty acid-induced Ca2+-dependent uncoupling and activation of external pathway of NADH oxidation are coupled to cyclosporin A-sensitive mitochondrial permeability transition",

abstract = "Permeabilization of inner mitochondrial membrane by palmitic acid in the presence of Ca2+ (cyclosporin A-sensitive stimulation of respiration, decrease of Δψ and high amplitude swelling) is accompanied by activation of the external pathway of NADH oxidation in liver mitochondria. The 'pore'-sealing agents (cyclosporin A, Mg2+ with ADP, and L-carnitine with ATP) are equally effective in preventing the induction of external pathway of NADH oxidation by Ca2+ with palmitate. However, activities of these agents are different in respect to recoupling of permeabilized mitochondria. Participation of cyclosporin A-sensitive 'pore' in the fatty acid- and Ca2+-dependent induction of external pathway of NADH oxidation and in Ca2+-dependent uncoupling is discussed.",

author = "Starkov, {A. A.} and Markova, {O. V.} and Mokhova, {E. N.} and E. Arrigoni-Martelli and Bobyleva, {V. A.}",

year = "1994",

language = "English (US)",

volume = "32",

pages = "1147--1155",

journal = "Biochemistry and Molecular Biology International",

issn = "1039-9712",

publisher = "John Wiley and Sons Inc.",

number = "6",

}

Fatty acid-induced Ca²⁺-dependent uncoupling and activation of external pathway of NADH oxidation are coupled to cyclosporin A-sensitive mitochondrial permeability transition. / Starkov, A. A.; Markova, O. V.; Mokhova, E. N. et al.
In: Biochemistry and Molecular Biology International, Vol. 32, No. 6, 1994, p. 1147-1155.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Fatty acid-induced Ca2+-dependent uncoupling and activation of external pathway of NADH oxidation are coupled to cyclosporin A-sensitive mitochondrial permeability transition

AU - Starkov, A. A.

AU - Markova, O. V.

AU - Mokhova, E. N.

AU - Arrigoni-Martelli, E.

AU - Bobyleva, V. A.

PY - 1994

Y1 - 1994

N2 - Permeabilization of inner mitochondrial membrane by palmitic acid in the presence of Ca2+ (cyclosporin A-sensitive stimulation of respiration, decrease of Δψ and high amplitude swelling) is accompanied by activation of the external pathway of NADH oxidation in liver mitochondria. The 'pore'-sealing agents (cyclosporin A, Mg2+ with ADP, and L-carnitine with ATP) are equally effective in preventing the induction of external pathway of NADH oxidation by Ca2+ with palmitate. However, activities of these agents are different in respect to recoupling of permeabilized mitochondria. Participation of cyclosporin A-sensitive 'pore' in the fatty acid- and Ca2+-dependent induction of external pathway of NADH oxidation and in Ca2+-dependent uncoupling is discussed.

AB - Permeabilization of inner mitochondrial membrane by palmitic acid in the presence of Ca2+ (cyclosporin A-sensitive stimulation of respiration, decrease of Δψ and high amplitude swelling) is accompanied by activation of the external pathway of NADH oxidation in liver mitochondria. The 'pore'-sealing agents (cyclosporin A, Mg2+ with ADP, and L-carnitine with ATP) are equally effective in preventing the induction of external pathway of NADH oxidation by Ca2+ with palmitate. However, activities of these agents are different in respect to recoupling of permeabilized mitochondria. Participation of cyclosporin A-sensitive 'pore' in the fatty acid- and Ca2+-dependent induction of external pathway of NADH oxidation and in Ca2+-dependent uncoupling is discussed.

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UR - http://www.scopus.com/inward/citedby.url?scp=0028235262&partnerID=8YFLogxK

M3 - Article

C2 - 8061632

AN - SCOPUS:0028235262

SN - 1039-9712

VL - 32

SP - 1147

EP - 1155

JO - Biochemistry and Molecular Biology International

JF - Biochemistry and Molecular Biology International

IS - 6

ER -

Fatty acid-induced Ca²⁺-dependent uncoupling and activation of external pathway of NADH oxidation are coupled to cyclosporin A-sensitive mitochondrial permeability transition

Abstract

All Science Journal Classification (ASJC) codes

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