TY - JOUR
T1 - Fish Probiotics
T2 - Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli
AU - Mirzabekyan, Susanna
AU - Harutyunyan, Natalya
AU - Manvelyan, Anahit
AU - Malkhasyan, Lilit
AU - Balayan, Marine
AU - Miralimova, Shakhlo
AU - Chikindas, Michael L.
AU - Chistyakov, Vladimir
AU - Pepoyan, Astghik
N1 - Funding Information:
This work was supported by the State Committee of Science, Armenia (project 21AG-4D065); by the Ministry of Innovative Development of the Republic of Uzbekistan, grant EAPI-2021-51; and by the Russian Foundation for Basic Research, project no. 20-516-81004\20.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/3
Y1 - 2023/3
N2 - The properties of intestinal bacteria/probiotics, such as cell surface hydrophobicity (CSH), auto-aggregation, and biofilm formation ability, play an important role in shaping the relationship between the bacteria and the host. The current study aimed to investigate the cell surface properties of fish intestinal bacteria and probiotics. Microbial adhesion to hydrocarbons was tested according to Kos and coauthors. The aggregation abilities of the investigated strains were studied as described by Collado and coauthors. The ability of bacterial isolates to form a biofilm was determined by performing a qualitative analysis using crystal violet staining based on the attachment of bacteria to polystyrene. These studies prove that bacterial cell surface hydrophobicity (CSH) is associated with the growth medium, and the effect of the growth medium on CSH is species-specific and likely also strain-specific. Isolates of intestinal lactobacilli from fish (Salmo ischchan) differed from isolates of non-fish/shrimp origin in the relationship between auto-aggregation and biofilm formation. Average CSH levels for fish lactobacilli and E. coli might were lower compared to those of non-fish origin, which may affect the efficiency of non-fish probiotics use in fisheries due to the peculiarities of the hosts’ aquatic lifestyles.
AB - The properties of intestinal bacteria/probiotics, such as cell surface hydrophobicity (CSH), auto-aggregation, and biofilm formation ability, play an important role in shaping the relationship between the bacteria and the host. The current study aimed to investigate the cell surface properties of fish intestinal bacteria and probiotics. Microbial adhesion to hydrocarbons was tested according to Kos and coauthors. The aggregation abilities of the investigated strains were studied as described by Collado and coauthors. The ability of bacterial isolates to form a biofilm was determined by performing a qualitative analysis using crystal violet staining based on the attachment of bacteria to polystyrene. These studies prove that bacterial cell surface hydrophobicity (CSH) is associated with the growth medium, and the effect of the growth medium on CSH is species-specific and likely also strain-specific. Isolates of intestinal lactobacilli from fish (Salmo ischchan) differed from isolates of non-fish/shrimp origin in the relationship between auto-aggregation and biofilm formation. Average CSH levels for fish lactobacilli and E. coli might were lower compared to those of non-fish origin, which may affect the efficiency of non-fish probiotics use in fisheries due to the peculiarities of the hosts’ aquatic lifestyles.
KW - auto-aggregation
KW - biofilm formation ability
KW - cell surface hydrophobicity
KW - E. coli
KW - fish
KW - lactic acid bacteria
KW - probiotic
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U2 - 10.3390/microorganisms11030595
DO - 10.3390/microorganisms11030595
M3 - Article
AN - SCOPUS:85151324296
SN - 2076-2607
VL - 11
JO - Microorganisms
JF - Microorganisms
IS - 3
M1 - 595
ER -