Flow cytometric detection of ornithine decarboxylase activity in epidermal cell subpopulations

Fredika M. Robertson; Susan K. Gilmour; Andrew J. Beavis; Sean M. O'Connell; Alan H. Conney; Mon‐Tuan ‐T Huang; Jeffrey D. Laskin; Oili A. Hietala; Thomas G. O'Brien

doi:10.1002/cyto.990110709

Flow cytometric detection of ornithine decarboxylase activity in epidermal cell subpopulations

Fredika M. Robertson, Susan K. Gilmour, Andrew J. Beavis, Sean M. O'Connell, Alan H. Conney, Mon‐Tuan ‐T Huang, Jeffrey D. Laskin, Oili A. Hietala, Thomas G. O'Brien

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Using flow cytometry in combination with membrane permeabilization techniques to enhance binding of antibodies with immunoreactive protein within the cytoplasm, we have developed a method to examine the ornithine decarboxylase (ODC) activity present within subpopulations of epidermal cells following acute and chronic exposure to the phorbol ester tumor promoter 12‐0‐tetradecanoylphorbol‐13‐acetate (TPA). The method described has the sensitivity to detect basal levels of ODC as well as increases in ODC at early time points following treatment with TPA and has the additional advantage of allowing subpopulation identification and characterization.

Original language	English (US)
Pages (from-to)	832-836
Number of pages	5
Journal	Cytometry
Volume	11
Issue number	7
DOIs	https://doi.org/10.1002/cyto.990110709
State	Published - 1990

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Biophysics
Hematology
Endocrinology
Cell Biology

Keywords

Phorbol ester
immunoreactive protein
proliferation marker

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10.1002/cyto.990110709

Cite this

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title = "Flow cytometric detection of ornithine decarboxylase activity in epidermal cell subpopulations",

abstract = "Using flow cytometry in combination with membrane permeabilization techniques to enhance binding of antibodies with immunoreactive protein within the cytoplasm, we have developed a method to examine the ornithine decarboxylase (ODC) activity present within subpopulations of epidermal cells following acute and chronic exposure to the phorbol ester tumor promoter 12‐0‐tetradecanoylphorbol‐13‐acetate (TPA). The method described has the sensitivity to detect basal levels of ODC as well as increases in ODC at early time points following treatment with TPA and has the additional advantage of allowing subpopulation identification and characterization.",

keywords = "Phorbol ester, immunoreactive protein, proliferation marker",

author = "Robertson, {Fredika M.} and Gilmour, {Susan K.} and Beavis, {Andrew J.} and O'Connell, {Sean M.} and Conney, {Alan H.} and Huang, {Mon‐Tuan ‐T} and Laskin, {Jeffrey D.} and Hietala, {Oili A.} and O'Brien, {Thomas G.}",

year = "1990",

doi = "10.1002/cyto.990110709",

language = "English (US)",

volume = "11",

pages = "832--836",

journal = "Cytometry",

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publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Flow cytometric detection of ornithine decarboxylase activity in epidermal cell subpopulations

AU - Robertson, Fredika M.

AU - Gilmour, Susan K.

AU - Beavis, Andrew J.

AU - O'Connell, Sean M.

AU - Conney, Alan H.

AU - Huang, Mon‐Tuan ‐T

AU - Laskin, Jeffrey D.

AU - Hietala, Oili A.

AU - O'Brien, Thomas G.

PY - 1990

Y1 - 1990

N2 - Using flow cytometry in combination with membrane permeabilization techniques to enhance binding of antibodies with immunoreactive protein within the cytoplasm, we have developed a method to examine the ornithine decarboxylase (ODC) activity present within subpopulations of epidermal cells following acute and chronic exposure to the phorbol ester tumor promoter 12‐0‐tetradecanoylphorbol‐13‐acetate (TPA). The method described has the sensitivity to detect basal levels of ODC as well as increases in ODC at early time points following treatment with TPA and has the additional advantage of allowing subpopulation identification and characterization.

AB - Using flow cytometry in combination with membrane permeabilization techniques to enhance binding of antibodies with immunoreactive protein within the cytoplasm, we have developed a method to examine the ornithine decarboxylase (ODC) activity present within subpopulations of epidermal cells following acute and chronic exposure to the phorbol ester tumor promoter 12‐0‐tetradecanoylphorbol‐13‐acetate (TPA). The method described has the sensitivity to detect basal levels of ODC as well as increases in ODC at early time points following treatment with TPA and has the additional advantage of allowing subpopulation identification and characterization.

KW - Phorbol ester

KW - immunoreactive protein

KW - proliferation marker

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DO - 10.1002/cyto.990110709

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SN - 0196-4763

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JO - Cytometry

JF - Cytometry

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Flow cytometric detection of ornithine decarboxylase activity in epidermal cell subpopulations

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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