Flow cytometric detection of ornithine decarboxylase activity in epidermal cell subpopulations

Fredika M. Robertson, Susan K. Gilmour, Andrew J. Beavis, Sean M. O'Connell, Alan H. Conney, Mon‐Tuan ‐T Huang, Jeffrey D. Laskin, Oili A. Hietala, Thomas G. O'Brien

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Using flow cytometry in combination with membrane permeabilization techniques to enhance binding of antibodies with immunoreactive protein within the cytoplasm, we have developed a method to examine the ornithine decarboxylase (ODC) activity present within subpopulations of epidermal cells following acute and chronic exposure to the phorbol ester tumor promoter 12‐0‐tetradecanoylphorbol‐13‐acetate (TPA). The method described has the sensitivity to detect basal levels of ODC as well as increases in ODC at early time points following treatment with TPA and has the additional advantage of allowing subpopulation identification and characterization.

Original languageEnglish (US)
Pages (from-to)832-836
Number of pages5
Issue number7
StatePublished - 1990

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology


  • Phorbol ester
  • immunoreactive protein
  • proliferation marker


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