Fourier Transform Infrared Microscopic Imaging: Effects of Estrogen and Estrogen Deficiency on Fracture Healing in Rat Femurs

Hao Ouyang, Pamela J. Sherman, Eleftherios P. Paschalis, Adele L. Boskey, Richard Mendelsohn

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Infrared spectroscopic imaging with 6-10 μm spatial resolution was used to characterize the changes in fracture callus mineral content, carbonate content, mineral crystallinity, and collagen maturity in femurs of 3-month-old ovariectomized rats treated with estrogen (estrogen sufficiency) or vehicle (estrogen deficiency). Comparisons were also made in these animals to cortical bone at a distance from the callus. Analyses at 4, 8, and 12 weeks post fracture demonstrated that healing was accelerated in the estrogen-sufficient animals as demonstrated by increasing mineral content and collagen maturity and decreasing carbonate incorporation.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalApplied Spectroscopy
Volume58
Issue number1
DOIs
StatePublished - Jan 2004

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Spectroscopy

Keywords

  • Estrogen therapy
  • Fracture healing
  • Infrared imaging
  • Infrared spectroscopy

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