Fourier Transform Infrared Microscopic Imaging: Effects of Estrogen and Estrogen Deficiency on Fracture Healing in Rat Femurs

Hao Ouyang; Pamela J. Sherman; Eleftherios P. Paschalis; Adele L. Boskey; Richard Mendelsohn

doi:10.1366/000370204322729405

Fourier Transform Infrared Microscopic Imaging: Effects of Estrogen and Estrogen Deficiency on Fracture Healing in Rat Femurs

Hao Ouyang, Pamela J. Sherman, Eleftherios P. Paschalis, Adele L. Boskey, Richard Mendelsohn

SASN Chemistry

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Infrared spectroscopic imaging with 6-10 μm spatial resolution was used to characterize the changes in fracture callus mineral content, carbonate content, mineral crystallinity, and collagen maturity in femurs of 3-month-old ovariectomized rats treated with estrogen (estrogen sufficiency) or vehicle (estrogen deficiency). Comparisons were also made in these animals to cortical bone at a distance from the callus. Analyses at 4, 8, and 12 weeks post fracture demonstrated that healing was accelerated in the estrogen-sufficient animals as demonstrated by increasing mineral content and collagen maturity and decreasing carbonate incorporation.

Original language	English (US)
Pages (from-to)	1-9
Number of pages	9
Journal	Applied Spectroscopy
Volume	58
Issue number	1
DOIs	https://doi.org/10.1366/000370204322729405
State	Published - Jan 2004

All Science Journal Classification (ASJC) codes

Instrumentation
Spectroscopy

Keywords

Estrogen therapy
Fracture healing
Infrared imaging
Infrared spectroscopy

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10.1366/000370204322729405

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title = "Fourier Transform Infrared Microscopic Imaging: Effects of Estrogen and Estrogen Deficiency on Fracture Healing in Rat Femurs",

abstract = "Infrared spectroscopic imaging with 6-10 μm spatial resolution was used to characterize the changes in fracture callus mineral content, carbonate content, mineral crystallinity, and collagen maturity in femurs of 3-month-old ovariectomized rats treated with estrogen (estrogen sufficiency) or vehicle (estrogen deficiency). Comparisons were also made in these animals to cortical bone at a distance from the callus. Analyses at 4, 8, and 12 weeks post fracture demonstrated that healing was accelerated in the estrogen-sufficient animals as demonstrated by increasing mineral content and collagen maturity and decreasing carbonate incorporation.",

keywords = "Estrogen therapy, Fracture healing, Infrared imaging, Infrared spectroscopy",

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AU - Sherman, Pamela J.

AU - Paschalis, Eleftherios P.

AU - Boskey, Adele L.

AU - Mendelsohn, Richard

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AB - Infrared spectroscopic imaging with 6-10 μm spatial resolution was used to characterize the changes in fracture callus mineral content, carbonate content, mineral crystallinity, and collagen maturity in femurs of 3-month-old ovariectomized rats treated with estrogen (estrogen sufficiency) or vehicle (estrogen deficiency). Comparisons were also made in these animals to cortical bone at a distance from the callus. Analyses at 4, 8, and 12 weeks post fracture demonstrated that healing was accelerated in the estrogen-sufficient animals as demonstrated by increasing mineral content and collagen maturity and decreasing carbonate incorporation.

KW - Estrogen therapy

KW - Fracture healing

KW - Infrared imaging

KW - Infrared spectroscopy

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Fourier Transform Infrared Microscopic Imaging: Effects of Estrogen and Estrogen Deficiency on Fracture Healing in Rat Femurs

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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