Fractionation of plant aminoacyl-tRNA synthetases on tRNA-sepharose columns

Hieronim Jakubowski, Jerzy Pawełkiewicz

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

It has been shown that tRNA-Sepharose, a chromatographic adsorbent containing unfractionated tRNA bound to a Sepharose matrix, is a useful, group-specific adsorbent for fractionation of the plant aminoacyl-tRNA synthetases. Conditions are described in which Val-, Trp-, Phe-, Leu- and Ile-tRNA synthetases from yellow lupin seeds can be separated from each other on the tRNA-Sepharose columns. Factors affecting affinity chromatography on the tRNA-Sepharose columns are discussed. The affinity chromatography procedure for the purification of lupin Ser-tRNA synthetase to homogenity is described.

Original languageEnglish (US)
Pages (from-to)213-221
Number of pages9
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume407
Issue number2
DOIs
StatePublished - Oct 1 1975
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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