TY - JOUR
T1 - Fractionation of plant aminoacyl-tRNA synthetases on tRNA-sepharose columns
AU - Jakubowski, Hieronim
AU - Pawełkiewicz, Jerzy
PY - 1975/10/1
Y1 - 1975/10/1
N2 - It has been shown that tRNA-Sepharose, a chromatographic adsorbent containing unfractionated tRNA bound to a Sepharose matrix, is a useful, group-specific adsorbent for fractionation of the plant aminoacyl-tRNA synthetases. Conditions are described in which Val-, Trp-, Phe-, Leu- and Ile-tRNA synthetases from yellow lupin seeds can be separated from each other on the tRNA-Sepharose columns. Factors affecting affinity chromatography on the tRNA-Sepharose columns are discussed. The affinity chromatography procedure for the purification of lupin Ser-tRNA synthetase to homogenity is described.
AB - It has been shown that tRNA-Sepharose, a chromatographic adsorbent containing unfractionated tRNA bound to a Sepharose matrix, is a useful, group-specific adsorbent for fractionation of the plant aminoacyl-tRNA synthetases. Conditions are described in which Val-, Trp-, Phe-, Leu- and Ile-tRNA synthetases from yellow lupin seeds can be separated from each other on the tRNA-Sepharose columns. Factors affecting affinity chromatography on the tRNA-Sepharose columns are discussed. The affinity chromatography procedure for the purification of lupin Ser-tRNA synthetase to homogenity is described.
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U2 - 10.1016/0005-2787(75)90286-5
DO - 10.1016/0005-2787(75)90286-5
M3 - Article
C2 - 1182179
AN - SCOPUS:0016714277
SN - 0005-2787
VL - 407
SP - 213
EP - 221
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 2
ER -