TY - JOUR
T1 - Frameshift mutations resulting in the changes of the same amino acid residue (140) in T4 bacteriophage lysozyme and in vivo codons for Trp, Tyr, Met, Val and Ile
AU - Tsugita, Akira
AU - Inouye, Masayori
AU - Imagawa, Tadashi
AU - Nakanishi, Tazumi
AU - Okada, Yoshimi
AU - Emrich, Joyce
AU - Streisinger, George
N1 - Funding Information:
The investigation was supported by grants to one of us (A.T.) from the Japanese Ministry of Education, from the Jane Coffin Childs Memorial Fund for Medical Research, U.S.A. and from the National Institutes of Health, U.S.A. (no. 5ROl GM10982), to another of the authors (G.S.) from the National Science Foundation (466 GB2261) and to another (Y.O.) from the Jane Coffin Childs Memorial Fund for Medical Research, U.S.A.
PY - 1969/5/14
Y1 - 1969/5/14
N2 - The lysozymes of three new strains of double frameshift mutants of T4 bacteriophage, eJ37eJD3, eJ200eJD4 and eJ25eJD1, were isolated from their respective lysates, and their amino acid sequences were compared with that of the wild-type strain. It was found that Trp138-Tyr-Asn140 in wild-type lysozyme changes to Met-Val-Tyr in eJ37eJD3, Tyr139-Asn140 to Cys-Ile-Ile in eJ200eJD4 and Asn140 to Ile-Ile in eJ25eJD1. From these results, a new hot spot for frameshift mutations has been found at Asn140 and in vivo codons for Trp, Tyr, Met, Val and Ile are thought to be UGG, UAU, AUG and AUA, respectively. Nineteen in vivo codons out of 64 codons have been identified by these results together with our previous results.
AB - The lysozymes of three new strains of double frameshift mutants of T4 bacteriophage, eJ37eJD3, eJ200eJD4 and eJ25eJD1, were isolated from their respective lysates, and their amino acid sequences were compared with that of the wild-type strain. It was found that Trp138-Tyr-Asn140 in wild-type lysozyme changes to Met-Val-Tyr in eJ37eJD3, Tyr139-Asn140 to Cys-Ile-Ile in eJ200eJD4 and Asn140 to Ile-Ile in eJ25eJD1. From these results, a new hot spot for frameshift mutations has been found at Asn140 and in vivo codons for Trp, Tyr, Met, Val and Ile are thought to be UGG, UAU, AUG and AUA, respectively. Nineteen in vivo codons out of 64 codons have been identified by these results together with our previous results.
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U2 - 10.1016/0022-2836(69)90281-2
DO - 10.1016/0022-2836(69)90281-2
M3 - Article
C2 - 5803289
AN - SCOPUS:0014674508
VL - 41
SP - 349
EP - 364
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 3
ER -