Frameshift mutations resulting in the changes of the same amino acid residue (140) in T4 bacteriophage lysozyme and in vivo codons for Trp, Tyr, Met, Val and Ile

Akira Tsugita; Masayori Inouye; Tadashi Imagawa; Tazumi Nakanishi; Yoshimi Okada; Joyce Emrich; George Streisinger

doi:10.1016/0022-2836(69)90281-2

Frameshift mutations resulting in the changes of the same amino acid residue (140) in T4 bacteriophage lysozyme and in vivo codons for Trp, Tyr, Met, Val and Ile

Akira Tsugita, Masayori Inouye, Tadashi Imagawa, Tazumi Nakanishi, Yoshimi Okada, Joyce Emrich, George Streisinger

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The lysozymes of three new strains of double frameshift mutants of T4 bacteriophage, eJ37eJD3, eJ200eJD4 and eJ25eJD1, were isolated from their respective lysates, and their amino acid sequences were compared with that of the wild-type strain. It was found that Trp¹³⁸-Tyr-Asn¹⁴⁰ in wild-type lysozyme changes to Met-Val-Tyr in eJ37eJD3, Tyr¹³⁹-Asn¹⁴⁰ to Cys-Ile-Ile in eJ200eJD4 and Asn¹⁴⁰ to Ile-Ile in eJ25eJD1. From these results, a new hot spot for frameshift mutations has been found at Asn¹⁴⁰ and in vivo codons for Trp, Tyr, Met, Val and Ile are thought to be UGG, UAU, AUG and AUA, respectively. Nineteen in vivo codons out of 64 codons have been identified by these results together with our previous results.

Original language	English (US)
Pages (from-to)	349-364
Number of pages	16
Journal	Journal of molecular biology
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/0022-2836(69)90281-2
State	Published - May 14 1969
Externally published	Yes

All Science Journal Classification (ASJC) codes

Structural Biology
Molecular Biology

Access to Document

10.1016/0022-2836(69)90281-2

Fingerprint Dive into the research topics of 'Frameshift mutations resulting in the changes of the same amino acid residue (140) in T4 bacteriophage lysozyme and in vivo codons for Trp, Tyr, Met, Val and Ile'. Together they form a unique fingerprint.

Cite this

@article{92a630ee85d24ca8b5c55ae026ba6c1a,

title = "Frameshift mutations resulting in the changes of the same amino acid residue (140) in T4 bacteriophage lysozyme and in vivo codons for Trp, Tyr, Met, Val and Ile",

abstract = "The lysozymes of three new strains of double frameshift mutants of T4 bacteriophage, eJ37eJD3, eJ200eJD4 and eJ25eJD1, were isolated from their respective lysates, and their amino acid sequences were compared with that of the wild-type strain. It was found that Trp138-Tyr-Asn140 in wild-type lysozyme changes to Met-Val-Tyr in eJ37eJD3, Tyr139-Asn140 to Cys-Ile-Ile in eJ200eJD4 and Asn140 to Ile-Ile in eJ25eJD1. From these results, a new hot spot for frameshift mutations has been found at Asn140 and in vivo codons for Trp, Tyr, Met, Val and Ile are thought to be UGG, UAU, AUG and AUA, respectively. Nineteen in vivo codons out of 64 codons have been identified by these results together with our previous results.",

author = "Akira Tsugita and Masayori Inouye and Tadashi Imagawa and Tazumi Nakanishi and Yoshimi Okada and Joyce Emrich and George Streisinger",

note = "Funding Information: The investigation was supported by grants to one of us (A.T.) from the Japanese Ministry of Education, from the Jane Coffin Childs Memorial Fund for Medical Research, U.S.A. and from the National Institutes of Health, U.S.A. (no. 5ROl GM10982), to another of the authors (G.S.) from the National Science Foundation (466 GB2261) and to another (Y.O.) from the Jane Coffin Childs Memorial Fund for Medical Research, U.S.A.",

year = "1969",

month = may,

day = "14",

doi = "10.1016/0022-2836(69)90281-2",

language = "English (US)",

volume = "41",

pages = "349--364",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

number = "3",

}

Frameshift mutations resulting in the changes of the same amino acid residue (140) in T4 bacteriophage lysozyme and in vivo codons for Trp, Tyr, Met, Val and Ile. / Tsugita, Akira; Inouye, Masayori; Imagawa, Tadashi; Nakanishi, Tazumi; Okada, Yoshimi; Emrich, Joyce; Streisinger, George.

In: Journal of molecular biology, Vol. 41, No. 3, 14.05.1969, p. 349-364.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Frameshift mutations resulting in the changes of the same amino acid residue (140) in T4 bacteriophage lysozyme and in vivo codons for Trp, Tyr, Met, Val and Ile

AU - Tsugita, Akira

AU - Inouye, Masayori

AU - Imagawa, Tadashi

AU - Nakanishi, Tazumi

AU - Okada, Yoshimi

AU - Emrich, Joyce

AU - Streisinger, George

N1 - Funding Information: The investigation was supported by grants to one of us (A.T.) from the Japanese Ministry of Education, from the Jane Coffin Childs Memorial Fund for Medical Research, U.S.A. and from the National Institutes of Health, U.S.A. (no. 5ROl GM10982), to another of the authors (G.S.) from the National Science Foundation (466 GB2261) and to another (Y.O.) from the Jane Coffin Childs Memorial Fund for Medical Research, U.S.A.

PY - 1969/5/14

Y1 - 1969/5/14

N2 - The lysozymes of three new strains of double frameshift mutants of T4 bacteriophage, eJ37eJD3, eJ200eJD4 and eJ25eJD1, were isolated from their respective lysates, and their amino acid sequences were compared with that of the wild-type strain. It was found that Trp138-Tyr-Asn140 in wild-type lysozyme changes to Met-Val-Tyr in eJ37eJD3, Tyr139-Asn140 to Cys-Ile-Ile in eJ200eJD4 and Asn140 to Ile-Ile in eJ25eJD1. From these results, a new hot spot for frameshift mutations has been found at Asn140 and in vivo codons for Trp, Tyr, Met, Val and Ile are thought to be UGG, UAU, AUG and AUA, respectively. Nineteen in vivo codons out of 64 codons have been identified by these results together with our previous results.

AB - The lysozymes of three new strains of double frameshift mutants of T4 bacteriophage, eJ37eJD3, eJ200eJD4 and eJ25eJD1, were isolated from their respective lysates, and their amino acid sequences were compared with that of the wild-type strain. It was found that Trp138-Tyr-Asn140 in wild-type lysozyme changes to Met-Val-Tyr in eJ37eJD3, Tyr139-Asn140 to Cys-Ile-Ile in eJ200eJD4 and Asn140 to Ile-Ile in eJ25eJD1. From these results, a new hot spot for frameshift mutations has been found at Asn140 and in vivo codons for Trp, Tyr, Met, Val and Ile are thought to be UGG, UAU, AUG and AUA, respectively. Nineteen in vivo codons out of 64 codons have been identified by these results together with our previous results.

UR - http://www.scopus.com/inward/record.url?scp=0014674508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014674508&partnerID=8YFLogxK

U2 - 10.1016/0022-2836(69)90281-2

DO - 10.1016/0022-2836(69)90281-2

M3 - Article

C2 - 5803289

AN - SCOPUS:0014674508

VL - 41

SP - 349

EP - 364

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 3

ER -