From adenylate cyclase to guanylate cyclase. Mutational analysis of a change in substrate specificity

Annie Beuve, Antoine Danchin

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Adenylate and guanylate cyclases, having different but related substrates, are a paradigm for the study of substrate discrimination. A prokaryotic adenylate cyclase gene, phylogenetically related to eukaryotic counterparts, was screened for mutants remodelling the enzyme's specificity. In a first step, a mutant was selected displaying a significant level of guanylate cyclase activity. This was due to a point mutation destroying most of the adenylate cyclase activity. A second selection step restored most of the original activity. This resulted from an additional mutation in the same region, thus permitting the first identification of a functional domain in adenylate and guanylate cyclases.

Original languageEnglish (US)
Pages (from-to)933-938
Number of pages6
JournalJournal of molecular biology
Volume225
Issue number4
DOIs
StatePublished - Jun 20 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Keywords

  • Rhizobium meliloti
  • adenylate cyclase
  • guanylate cyclase
  • substrate specificity
  • β-galactosidase fusion

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