Abstract
Adenylate and guanylate cyclases, having different but related substrates, are a paradigm for the study of substrate discrimination. A prokaryotic adenylate cyclase gene, phylogenetically related to eukaryotic counterparts, was screened for mutants remodelling the enzyme's specificity. In a first step, a mutant was selected displaying a significant level of guanylate cyclase activity. This was due to a point mutation destroying most of the adenylate cyclase activity. A second selection step restored most of the original activity. This resulted from an additional mutation in the same region, thus permitting the first identification of a functional domain in adenylate and guanylate cyclases.
Original language | English (US) |
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Pages (from-to) | 933-938 |
Number of pages | 6 |
Journal | Journal of molecular biology |
Volume | 225 |
Issue number | 4 |
DOIs | |
State | Published - Jun 20 1992 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology
Keywords
- Rhizobium meliloti
- adenylate cyclase
- guanylate cyclase
- substrate specificity
- β-galactosidase fusion