@inbook{1363e735cab2491091d3734fccede35d,
title = "From Sequences to Enzymes: Heterologous Expression of Genes from Marine Microbes",
abstract = "Heterologous expression is an easy and broadly applicable experimental approach widely used to investigate protein functions without the need to genetically manipulate the original host. The approach is used to obtain large quantities of the desired protein, which can be further analyzed from a biochemical, structural, and functional perspective. The expression system consists of three main components: (1) a foreign DNA sequence coding for the protein of interest; (2) a suitable expression vector; (3) a suitable host (bacterial, yeast, or mammalian cells) which does not encode or express the protein of interest. Here, we show how to apply an Escherichia coli-based expression system to overexpress protein encoding genes from marine microbes.",
keywords = "E. coli, Expression vector, Heterologous expression, Metagenome, Recombinant protein, Uncultured microorganism",
author = "Angelica Severino and Alessandro Coppola and Monica Correggia and Costantino Vetriani and Donato Giovannelli and Angelina Cordone",
note = "Funding Information: This work was partially supported by the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation programme (grant agreement No. 948972), NSF grant IOS 19-51690, and NASA grant 80NSSC21K0485. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2313-8_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "265--281",
booktitle = "Methods in Molecular Biology",
}