From Sequences to Enzymes: Heterologous Expression of Genes from Marine Microbes

Angelica Severino, Alessandro Coppola, Monica Correggia, Costantino Vetriani, Donato Giovannelli, Angelina Cordone

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Heterologous expression is an easy and broadly applicable experimental approach widely used to investigate protein functions without the need to genetically manipulate the original host. The approach is used to obtain large quantities of the desired protein, which can be further analyzed from a biochemical, structural, and functional perspective. The expression system consists of three main components: (1) a foreign DNA sequence coding for the protein of interest; (2) a suitable expression vector; (3) a suitable host (bacterial, yeast, or mammalian cells) which does not encode or express the protein of interest. Here, we show how to apply an Escherichia coli-based expression system to overexpress protein encoding genes from marine microbes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages265-281
Number of pages17
DOIs
StatePublished - 2022
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2498
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • E. coli
  • Expression vector
  • Heterologous expression
  • Metagenome
  • Recombinant protein
  • Uncultured microorganism

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