Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein

Aline Tabib-Salazar, Bing Liu, Andrey Shadrin, Lynn Burchell, Zhexin Wang, Zhihao Wang, Moran G. Goren, Ido Yosef, Udi Qimron, Konstantin Severinov, Steve J. Matthews, Sivaramesh Wigneshweraraj

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Infection of Escherichia coli by the T7 phage leads to rapid and selective inhibition of the bacterial RNA polymerase (RNAP) by the 7 kDa T7 protein Gp2. We describe the identification and functional and structural characterisation of a novel 7 kDa T7 protein, Gp5.7, which adopts a winged helix-turn-helix-like structure and specifically represses transcription initiation from host RNAP-dependent promoters on the phage genome via a mechanism that involves interaction with DNA and the bacterial RNAP. Whereas Gp2 is indispensable for T7 growth in E. coli, we show that Gp5.7 is required for optimal infection outcome. Our findings provide novel insights into how phages fine-tune the activity of the host transcription machinery to ensure both successful and efficient phage progeny development.

Original languageEnglish (US)
Pages (from-to)7697-7707
Number of pages11
JournalNucleic acids research
Volume45
Issue number13
DOIs
StatePublished - Jul 1 2017

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Bacteriophage T7
DNA-Binding Proteins
DNA-Directed RNA Polymerases
Bacterial RNA
Bacteriophages
Escherichia coli
Coliphages
Infection
Proteins
Genome
DNA
Growth

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Tabib-Salazar, A., Liu, B., Shadrin, A., Burchell, L., Wang, Z., Wang, Z., ... Wigneshweraraj, S. (2017). Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein. Nucleic acids research, 45(13), 7697-7707. https://doi.org/10.1093/nar/gkx370
Tabib-Salazar, Aline ; Liu, Bing ; Shadrin, Andrey ; Burchell, Lynn ; Wang, Zhexin ; Wang, Zhihao ; Goren, Moran G. ; Yosef, Ido ; Qimron, Udi ; Severinov, Konstantin ; Matthews, Steve J. ; Wigneshweraraj, Sivaramesh. / Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein. In: Nucleic acids research. 2017 ; Vol. 45, No. 13. pp. 7697-7707.
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Tabib-Salazar, A, Liu, B, Shadrin, A, Burchell, L, Wang, Z, Wang, Z, Goren, MG, Yosef, I, Qimron, U, Severinov, K, Matthews, SJ & Wigneshweraraj, S 2017, 'Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein', Nucleic acids research, vol. 45, no. 13, pp. 7697-7707. https://doi.org/10.1093/nar/gkx370

Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein. / Tabib-Salazar, Aline; Liu, Bing; Shadrin, Andrey; Burchell, Lynn; Wang, Zhexin; Wang, Zhihao; Goren, Moran G.; Yosef, Ido; Qimron, Udi; Severinov, Konstantin; Matthews, Steve J.; Wigneshweraraj, Sivaramesh.

In: Nucleic acids research, Vol. 45, No. 13, 01.07.2017, p. 7697-7707.

Research output: Contribution to journalArticle

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AU - Tabib-Salazar, Aline

AU - Liu, Bing

AU - Shadrin, Andrey

AU - Burchell, Lynn

AU - Wang, Zhexin

AU - Wang, Zhihao

AU - Goren, Moran G.

AU - Yosef, Ido

AU - Qimron, Udi

AU - Severinov, Konstantin

AU - Matthews, Steve J.

AU - Wigneshweraraj, Sivaramesh

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