Functional Characterization of Drosophila Sialyltransferase

Kate Koles, Kenneth Irvine, Vladislav M. Panin

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Sialylation is an important carbohydrate modification of glycoconjugates in the deuterostome lineage of animals. By contrast, the evidence for sialylation in protostomes has been scarce and somewhat controversial. In the present study, we characterize a Drosophila sialyl-transferase gene, thus providing experimental evidence for the presence of sialylation in protostomes. This gene encodes a functional α2-6-sialyltransferase (SiaT) that is closely related to the vertebrate ST6Gal sialyltransferase family, indicating an ancient evolutionary origin for this family. Characterization of recombinant, purified Drosophila SiaT revealed a novel acceptor specificity as it exhibits highest activity toward GalNAcβ1-4GlcNAc carbohydrate structures at the non-reducing termini of oligosaccharides and glycoprotein glycans. Oligosaccharides are preferred over glycoproteins as acceptors, and no activity toward glycolipid acceptors was detected. Recombinant Drosophila SiaT expressed in cultured insect cells possesses in vivo and in vitro autosialylation activity toward β-linked GalNAc termini of its own N-linked glycans, thus representing the first example of a sialylated insect glycoconjugate. In situ hybridization revealed that Drosophila SiaT is expressed during embryonic development in a tissue- and stage-specific fashion, with elevated expression in a subset of cells within the central nervous system. The identification of a SiaT in Drosophila provides a new evolutionary perspective for considering the diverse functions of sialylation and, through the powerful genetic tools available in this system, a means of elucidating functions for sialylation in protostomes.

Original languageEnglish (US)
Pages (from-to)4346-4357
Number of pages12
JournalJournal of Biological Chemistry
Volume279
Issue number6
DOIs
StatePublished - Feb 6 2004

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Sialyltransferases
Drosophila
Glycoconjugates
Oligosaccharides
Polysaccharides
Insects
Glycoproteins
Genes
Carbohydrates
Glycolipids
Neurology
Transferases
Embryonic Development
In Situ Hybridization
Vertebrates
Cultured Cells
Animals
Central Nervous System
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Koles, Kate ; Irvine, Kenneth ; Panin, Vladislav M. / Functional Characterization of Drosophila Sialyltransferase. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 6. pp. 4346-4357.
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Functional Characterization of Drosophila Sialyltransferase. / Koles, Kate; Irvine, Kenneth; Panin, Vladislav M.

In: Journal of Biological Chemistry, Vol. 279, No. 6, 06.02.2004, p. 4346-4357.

Research output: Contribution to journalArticle

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