Functional characterization of the RNA-binding domain and motif of the double-stranded RNA-dependent protein kinase DAI (PKR)

Christian Schmedt, Simon R. Green, Lisa Manche, Deborah R. Taylor, Yuliang Ma, Michael B. Mathews

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

The double-stranded (ds) RNA-activated protein kinase, DAI (also known as PKR), contains an RNA-binding domain comprising two tandem repeats of a motif, the dsRBM, which is shared with a number of other proteins that interact with structured RNAs. We have expressed the entire domain and the first copy of the motif in Escherichia coli and purified the two proteins, p20 and p10, to apparent homogeneity in order to study their interactions with RNA and with the intact kinase enzyme. Both p20 and p10 bound preferentially to structured RNA molecules. Competition assays showed that in both cases the order of affinity is dsRNA > VA RNA > tRNA, but the isolated motif bound much less tightly than the entire domain. Measurement of the dissociation constants for dsRNA by quantitative gel mobility shift analysis gave apparent K(d) values of 4 × 10−9 M and 3.8 x 10−7 M for p20 and p10, respectively. The binding of p20 molecules to dsRNA appeared to be cooperative. Multiple complexes were formed between the intact domain and dsRNA, saturating at a density of about one p20 molecule/11.25 base-pairs (or one turn) of duplex, whereas p10 achieved only about half of this packing density. The apparent K(d) for the p20-VA RNA interaction was estimated as 3.5 × 10−7 M and at least three complexes were detected, but no distinct complexes were visualized for the interaction between p10 and VA RNA. Both p20 and p10 inhibited autophosphorylation of intact DAI, probably by binding the dsRNA activator. Once activated, DAI could phosphorylate both p10 and p20, suggesting that intermolecular phosphorylation can occur.

Original languageEnglish (US)
Pages (from-to)29-44
Number of pages16
JournalJournal of molecular biology
Volume249
Issue number1
DOIs
StatePublished - May 26 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Keywords

  • Adenovirus VA RNA
  • Dsrna
  • Protein kinase
  • RNA-binding motif
  • RNA-protein interactions

Fingerprint Dive into the research topics of 'Functional characterization of the RNA-binding domain and motif of the double-stranded RNA-dependent protein kinase DAI (PKR)'. Together they form a unique fingerprint.

Cite this