Functional interaction between RNA polymerase α subunit C-terminal domain and σ70 in UP-element- and activator-dependent transcription

Hao Chen, Hong Tang, Richard H. Ebright

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


We show that the Escherichia coli RNA polymerase (RNAP) α subunit C-terminal domain (αCTD) functionally interacts with σ70 at a subset of UP-element- and activator-dependent promoters, we define the determinants of αCTD and σ70 required for the interaction, and we present a structural model for the interaction. The αCTD-σ70 interaction spans the upstream promoter and core promoter, thereby linking recognition of UP-elements and activators in the upstream promoter with recognition of the -35 element in the core promoter. We propose that the αCTD-σ70 interaction permits UP-elements and activators not only to "recruit" RNAP through direct interaction with αCTD, but also to "remodel" RNAP-core-promoter interaction through indirect, αCTD-bridged interactions with σ70.

Original languageEnglish (US)
Pages (from-to)1621-1633
Number of pages13
JournalMolecular cell
Issue number6
StatePublished - Jun 1 2003

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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