Functional organization of the riboflavin biosynthesis operon from Bacillus subtilis SHgw

Vladimir N. Mironov, Alexander S. Kraev, Michael L. Chikindas, Boris K. Chernov, Anatoly I. Stepanov, Konstantin G. Skryabin

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

We have sequenced 6006 by DNA of a region from the Bacillus subtilis SHgw chromosome known to contain riboflavin biosynthesis genes (rib gene cluster, 210° on the B. subtilis genetic map). Five of the seven open reading frames found within the sequence are shown to represent the genes ribG, ribB, ribA, ribH and rib7D. The calculated molecular masses for the putative translation products are 39305, 23481, 44121, 16287 and 14574 daltons respectively. The five rib genes are transcribed as a polycistronic 4277 nucleotide messenger RNA. The steady-state level of the transcript is negatively regulated by riboflavin. A cis-acting element necessary for regulation was mapped by analysis of constitutive mutations within the 5′ untranslated region of the operon. The element is at least 48 by in length and does not bear obvious similarity to well defined prokaryotic regulatory elements. The molecular mechanism of regulation remains unknown, but the data presented argue against regulation by attenuation.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalMgg Molecular & General Genetics
Volume242
Issue number2
DOIs
StatePublished - Jan 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Bacillus subtilis
  • Expression regulation
  • Mutational analysis
  • Riboflavin operon
  • Transcript mapping

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