Further biochemical data on Qa-2

James Michaelson, Lorraine Flaherty, Yuri Bushkin, Holly Yudkowitz

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47 Scopus citations

Abstract

The Qa-2 differentiation alloantigen is coded by a gene situated between the D and Tla loci of the murine major histocompatibility complex (H-2). Qa-2-bearing protein was isolated by immunoprecipitation and found to be composed of subunits of 40 000 and 12 000 daltons by SDS polyacrylamide gel electrophoresis (PAGE). The 12 000 dalton material was identified as β2-microglobulin (β2M) by its molecular weight (SDS PAGE), charge (isoelectric focusing), antigenicity (reactivity with xenogenic anti-β 2M), and genetics. The 40 000 dalton mol. wt. of Qa-2 heavy chain is 5 000 daltons less than that of D and K molecules (45 000 daltons). The quantity of Qa-2 isolated by immunoprecipitation was found to vary in a strain-specific fashion and as much as a 15-fold difference was observed.

Original languageEnglish (US)
Pages (from-to)129-140
Number of pages12
JournalImmunogenetics
Volume14
Issue number1-2
DOIs
StatePublished - Sep 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

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