Fusion-complementation analysis of auxotrophic and chlorophyll-deficient lines isolated in haploid Nicotiana plumbaginifolia protoplast cultures

Vladimir A. Sidorov; Pal Maliga

doi:10.1007/BF00729450

Fusion-complementation analysis of auxotrophic and chlorophyll-deficient lines isolated in haploid Nicotiana plumbaginifolia protoplast cultures

Vladimir A. Sidorov, Pal Maliga

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21 Scopus citations

Abstract

The spontaneous frequency of pigment-deficient mutants was 5,7×10^-5 in haploid protoplast cultures of Nicotiana plumbaginifolia. This frequency could be increased to 8×10^-3 by⁶⁰Co irradiation. In the same system an isoleucine (ILE401), a uracil (URA401) and a leucine (LEU403) auxotroph were recovered by individual testing. Pigment deficiency (5 clones tested) and the auxotrophic traits were shown to be recessive by fusion-complementation. The suitability of haploid Nicotiana plumbaginifolia is discussed for studies on mutation induction in cultured cells.

Original language	English (US)
Pages (from-to)	328-332
Number of pages	5
Journal	Mgg Molecular & General Genetics
Volume	186
Issue number	3
DOIs	https://doi.org/10.1007/BF00729450
State	Published - Sep 1982
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

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10.1007/BF00729450

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title = "Fusion-complementation analysis of auxotrophic and chlorophyll-deficient lines isolated in haploid Nicotiana plumbaginifolia protoplast cultures",

abstract = "The spontaneous frequency of pigment-deficient mutants was 5,7×10-5 in haploid protoplast cultures of Nicotiana plumbaginifolia. This frequency could be increased to 8×10-3 by60Co irradiation. In the same system an isoleucine (ILE401), a uracil (URA401) and a leucine (LEU403) auxotroph were recovered by individual testing. Pigment deficiency (5 clones tested) and the auxotrophic traits were shown to be recessive by fusion-complementation. The suitability of haploid Nicotiana plumbaginifolia is discussed for studies on mutation induction in cultured cells.",

author = "Sidorov, {Vladimir A.} and Pal Maliga",

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T1 - Fusion-complementation analysis of auxotrophic and chlorophyll-deficient lines isolated in haploid Nicotiana plumbaginifolia protoplast cultures

AU - Sidorov, Vladimir A.

AU - Maliga, Pal

PY - 1982/9

Y1 - 1982/9

N2 - The spontaneous frequency of pigment-deficient mutants was 5,7×10-5 in haploid protoplast cultures of Nicotiana plumbaginifolia. This frequency could be increased to 8×10-3 by60Co irradiation. In the same system an isoleucine (ILE401), a uracil (URA401) and a leucine (LEU403) auxotroph were recovered by individual testing. Pigment deficiency (5 clones tested) and the auxotrophic traits were shown to be recessive by fusion-complementation. The suitability of haploid Nicotiana plumbaginifolia is discussed for studies on mutation induction in cultured cells.

AB - The spontaneous frequency of pigment-deficient mutants was 5,7×10-5 in haploid protoplast cultures of Nicotiana plumbaginifolia. This frequency could be increased to 8×10-3 by60Co irradiation. In the same system an isoleucine (ILE401), a uracil (URA401) and a leucine (LEU403) auxotroph were recovered by individual testing. Pigment deficiency (5 clones tested) and the auxotrophic traits were shown to be recessive by fusion-complementation. The suitability of haploid Nicotiana plumbaginifolia is discussed for studies on mutation induction in cultured cells.

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Fusion-complementation analysis of auxotrophic and chlorophyll-deficient lines isolated in haploid Nicotiana plumbaginifolia protoplast cultures

Abstract

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