Fusion-complementation analysis of auxotrophic and chlorophyll-deficient lines isolated in haploid Nicotiana plumbaginifolia protoplast cultures

Vladimir A. Sidorov, Pal Maliga

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The spontaneous frequency of pigment-deficient mutants was 5,7×10-5 in haploid protoplast cultures of Nicotiana plumbaginifolia. This frequency could be increased to 8×10-3 by60Co irradiation. In the same system an isoleucine (ILE401), a uracil (URA401) and a leucine (LEU403) auxotroph were recovered by individual testing. Pigment deficiency (5 clones tested) and the auxotrophic traits were shown to be recessive by fusion-complementation. The suitability of haploid Nicotiana plumbaginifolia is discussed for studies on mutation induction in cultured cells.

Original languageEnglish (US)
Pages (from-to)328-332
Number of pages5
JournalMgg Molecular & General Genetics
Volume186
Issue number3
DOIs
StatePublished - Sep 1 1982
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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