Abstract
Terbutryn-resistant plastids of the Nicotiana plumbaginifolia TBR2 mutant were introduced into N. tabacum plants by protoplast fusion following X-irradiation of TBR2 protoplasts. The N. tabacum chloroplast recipient line, SR1-A15, carried mutant (albino) plastids. Following protoplast fusion, potential cybrid cell lines with an N. tabacum (SR1-A15) nucleus and N. plumbaginifolia (TBR2) chloroplasts were identified by their green color. The presence of TBR2 plastids in regenerated green N. tabacum plants was confirmed by hybridization with a chloroplast DNA probe and by the modified chloroplast fluorescence transients characteristic of the TBR2 mutant. Cybrid plants were resistant to high levels of atrazine (10 kg/ha). The protruding stigma and shorter than normal filaments of the cybrids resulted in male sterility. In the cybrids atrazine resistance was associated with reduced vigour, suggesting a causal relationship.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 201-205 |
| Number of pages | 5 |
| Journal | Mgg Molecular & General Genetics |
| Volume | 205 |
| Issue number | 2 |
| DOIs | |
| State | Published - Nov 1986 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Genetics
Keywords
- Atrazine resistance
- Cytoplasmic male sterility
- Protoplast fusion