GABA receptors in the phrenic nucleus of the rat

V. C. Chitravanshi, Hreday Sapru

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The phrenic nucleus was identified by microinjections of N-methyl-D- aspartic acid in urethan-anesthetized adult male Wistar rats. Microinjections of GABA(A) and GABA(B) receptor agonists (muscimol and baclofen, respectively) at the same site decreased the phrenic nerve burst amplitude. Microinjections of GABA(A) and GABA(B) receptor antagonists (bicuculline and 2-hydroxysaclofen, respectively) blocked as well as reversed the effects of their respective agonists. These results were confirmed by recording extracellular action potentials from single phrenic neurons. Micropressure applications of muscimol and baclofen decreased the activity of single neurons in the phrenic nucleus; this effect was blocked as well as reversed by micropressure applications of bicuculline and 2-hydroxysaclofen, respectively. These results demonstrated the presence of GABA receptors on the neurons in the phrenic nucleus and suggested that their activation results in the decrease of the phrenic nerve burst amplitude. The importance of these results in the identification of neural circuits mediating inhibition of phrenic neurons is discussed.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume276
Issue number2 45-2
StatePublished - Feb 1 1999

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GABA Receptors
Microinjections
Neurons
Muscimol
Phrenic Nerve
Baclofen
Bicuculline
Diaphragm
gamma-Aminobutyric Acid
GABA-B Receptor Antagonists
GABA-B Receptor Agonists
Urethane
N-Methylaspartate
Action Potentials
Wistar Rats
Cervical Cord
2-hydroxysaclofen

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

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GABA receptors in the phrenic nucleus of the rat. / Chitravanshi, V. C.; Sapru, Hreday.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 276, No. 2 45-2, 01.02.1999.

Research output: Contribution to journalArticle

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