Galactose biosensors using composite polymers to prevent interferences

Paul Manowitz; Paul W. Stoecker; Alexander M. Yacynych

doi:10.1016/0956-5663(95)96854-R

Galactose biosensors using composite polymers to prevent interferences

Paul Manowitz, Paul W. Stoecker, Alexander M. Yacynych

Robert Wood Johnson Medical School (RWJMS), Psychiatry

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

A biosensor using a composite polymer to prevent interferences was used in a flow injection analysis system for the detection of galactose in human plasma. The biosensor consisted of galactose oxidase immobilized on a platinized carbon electrode that had been modified with a composite polymer. The composite polymer showed improved selectivity to hydrogen peroxide compared with either of its individual polymeric components, Nafion and a copolymer of diaminobenzene and resorcinol. The composite polymer minimized the effect of possible interference from urate, ascorbate, and acetaminophen. This analytical system has a minimum detection limit of 50 μM, linearity to 6 mM, a storage stability of greater than 30 days, and a high sample throughput (approx. 120 samples/h).

Original language	English (US)
Pages (from-to)	359-370
Number of pages	12
Journal	Biosensors and Bioelectronics
Volume	10
Issue number	3-4
DOIs	https://doi.org/10.1016/0956-5663(95)96854-R
State	Published - 1995

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

Keywords

1
3-diaminobenzene
3-phenylenediamine
Nafion
biosensor
composite polymer
electropolymerization
galactose
galactose oxidase
interferences

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10.1016/0956-5663(95)96854-R

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title = "Galactose biosensors using composite polymers to prevent interferences",

abstract = "A biosensor using a composite polymer to prevent interferences was used in a flow injection analysis system for the detection of galactose in human plasma. The biosensor consisted of galactose oxidase immobilized on a platinized carbon electrode that had been modified with a composite polymer. The composite polymer showed improved selectivity to hydrogen peroxide compared with either of its individual polymeric components, Nafion and a copolymer of diaminobenzene and resorcinol. The composite polymer minimized the effect of possible interference from urate, ascorbate, and acetaminophen. This analytical system has a minimum detection limit of 50 μM, linearity to 6 mM, a storage stability of greater than 30 days, and a high sample throughput (approx. 120 samples/h).",

keywords = "1, 3-diaminobenzene, 3-phenylenediamine, Nafion, biosensor, composite polymer, electropolymerization, galactose, galactose oxidase, interferences",

author = "Paul Manowitz and Stoecker, {Paul W.} and Yacynych, {Alexander M.}",

note = "Funding Information: The authors would like to acknowledge Dr Gad Avigad, Dr Stanley Stein, Dr Ronald Poretz, and Henry Lackland for their helpful suggestions. This work was supported by grants from the UNICO Foundation and the National Institute on Alcohol Abuse and Alcoholism Grant No. R01-AA07799.",

year = "1995",

doi = "10.1016/0956-5663(95)96854-R",

language = "English (US)",

volume = "10",

pages = "359--370",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Limited",

number = "3-4",

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TY - JOUR

T1 - Galactose biosensors using composite polymers to prevent interferences

AU - Manowitz, Paul

AU - Stoecker, Paul W.

AU - Yacynych, Alexander M.

N1 - Funding Information: The authors would like to acknowledge Dr Gad Avigad, Dr Stanley Stein, Dr Ronald Poretz, and Henry Lackland for their helpful suggestions. This work was supported by grants from the UNICO Foundation and the National Institute on Alcohol Abuse and Alcoholism Grant No. R01-AA07799.

PY - 1995

Y1 - 1995

N2 - A biosensor using a composite polymer to prevent interferences was used in a flow injection analysis system for the detection of galactose in human plasma. The biosensor consisted of galactose oxidase immobilized on a platinized carbon electrode that had been modified with a composite polymer. The composite polymer showed improved selectivity to hydrogen peroxide compared with either of its individual polymeric components, Nafion and a copolymer of diaminobenzene and resorcinol. The composite polymer minimized the effect of possible interference from urate, ascorbate, and acetaminophen. This analytical system has a minimum detection limit of 50 μM, linearity to 6 mM, a storage stability of greater than 30 days, and a high sample throughput (approx. 120 samples/h).

AB - A biosensor using a composite polymer to prevent interferences was used in a flow injection analysis system for the detection of galactose in human plasma. The biosensor consisted of galactose oxidase immobilized on a platinized carbon electrode that had been modified with a composite polymer. The composite polymer showed improved selectivity to hydrogen peroxide compared with either of its individual polymeric components, Nafion and a copolymer of diaminobenzene and resorcinol. The composite polymer minimized the effect of possible interference from urate, ascorbate, and acetaminophen. This analytical system has a minimum detection limit of 50 μM, linearity to 6 mM, a storage stability of greater than 30 days, and a high sample throughput (approx. 120 samples/h).

KW - 1

KW - 3-diaminobenzene

KW - 3-phenylenediamine

KW - Nafion

KW - biosensor

KW - composite polymer

KW - electropolymerization

KW - galactose

KW - galactose oxidase

KW - interferences

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U2 - 10.1016/0956-5663(95)96854-R

DO - 10.1016/0956-5663(95)96854-R

M3 - Article

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AN - SCOPUS:0029256699

SN - 0956-5663

VL - 10

SP - 359

EP - 370

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 3-4

ER -

Galactose biosensors using composite polymers to prevent interferences

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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