J. W. Fong, Robert Ledeen, S. K. Kundu, S. W. Brostoff

Research output: Contribution to journalArticle

58 Scopus citations


—Gangliosides have been isolated from myelin obtained from three types of peripheral nerve: bovine spinal roots, bovine sciatic nerve and human sciatic nerve. Yields in most cases were 218–287 μg of lipid‐bound sialic acid per g myelin, less than half that previously obtained from CNS myelin. Myelin accounted for approx 60% of total ganglioside present in whole spinal root. The human sample contained only N‐acetylneuraminic acid but the two bovine preparations contained that as well as N‐glycolylneuraminic acid; N‐acetylglucosamine and N‐acetylgalactosamine were both present in all three preparations. Sphingosine was the major long‐chain base in each preparation while 4‐eicosasphingenine (d20:1) comprised about 14% in the two bovine samples and 3% in the human sample. The major fatty acids in all preparations were 16:0, 18:0, 22:0, 24:0 and 24:1. Sialosylgalactosyl ceramide (G7), a ganglioside characteristic of CNS myelin, was not detected in any of the PNS samples. The majority of gangliosides in bovine spinal root myelin were monosialo species, although the structures differed in some respects from those of CNS myelin. The molar concentration of lipid‐bound sialic acid in PNS myelin is roughly equivalent to that of the P1 basic protein.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalJournal of neurochemistry
Issue number1
StatePublished - Jan 1 1976
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'GANGLIOSIDES OF PERIPHERAL NERVE MYELIN'. Together they form a unique fingerprint.

  • Cite this