Gel mapping of wheat and lupin tRNAs.

Z. A. Janowicz, H. Augustyniak, W. Kedzierski, H. Jakubowski, J. Augustyniak

Research output: Contribution to journalArticlepeer-review

Abstract

Transfer RNAs were isolated from plants representing mono- and dicotyledons: wheat embryos and lupin seeds. The two tRNA preparations were compared by polyacrylamide-gel-electrophoresis mapping. Transfer RNAs extracted from the separated spots were tested for acceptance of 11 amino acids. Comparison of electrophoretic mobility of respective tRNA species indicates the overall similarity of tRNA populations in the two plants studied. Especially, isoaccepting tRNAs for glycine, tyrosine and valine and some of isoacceptors of tRNAArg, tRNAAsp, tRNALeu, tRNALys and tRNAPhe occupy identical or closely similar positions on both polyacrylamide-gel maps. However, some tRNA isoacceptors from one population have no counterparts in the second one, which may indicate differences in their primary structures.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalActa biochimica Polonica
Volume28
Issue number2
StatePublished - 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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