Gene expression analysis of nuclear factor I-A deficient mice indicates delayed brain maturation

Yong Wong, Christian Schulze, Thomas Streichert, Richard M. Gronostajski, Melitta Camartin, Thomas Tilling

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Nuclear factor I-A (NFI-A), a phylogenetically conserved transcription/replication protein, plays a crucial role in mouse brain development. Previous studies have shown that disruption of the Nfia gene in mice leads to perinatal lethality, corpus callosum agenesis, and hydrocephalus. Results: To identify potential NFI-A target genes involved in the observed tissue malformations, we analyzed gene expression in brains from Nfia-/- and Nfia+/+ littermate mice at the mRNA level using oligonucleotide microarrays. In young postnatal animals (postnatal day 16), 356 genes were identified as being differentially regulated, whereas at the late embryonic stage (embryonic day 18) only five dysregulated genes were found. An in silico analysis identified phylogenetically conserved NFI binding sites in at least 70 of the differentially regulated genes. Moreover, assignment of gene function showed that marker genes for immature neural cells and neural precursors were expressed at elevated levels in young postnatal Nfia-/- mice. In contrast, marker genes for differentiated neural cells were downregulated at this stage. In particular, genes relevant for oligodendrocyte differentiation were affected. Conclusion: Our findings suggest that brain development, especially oligodendrocyte maturation, is delayed in Nfia-/- mice during the early postnatal period, which at least partly accounts for their phenotype. The identification of potential NFI-A target genes in our study should help to elucidate NFI-A dependent transcriptional pathways and contribute to enhanced understanding of this period of brain formation, especially with regard to the function of NFI-A.

Original languageEnglish (US)
Article numberR72
JournalGenome biology
Volume8
Issue number5
DOIs
StatePublished - May 2 2007

Fingerprint

NFI Transcription Factors
gene expression
brain
maturation
Gene Expression
gene
mice
Brain
Genes
genes
Oligodendroglia
neurons
hydrocephalus
genetic markers
postpartum period
gene targeting
oligonucleotides
analysis
Agenesis of Corpus Callosum
binding sites

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

Cite this

Wong, Yong ; Schulze, Christian ; Streichert, Thomas ; Gronostajski, Richard M. ; Camartin, Melitta ; Tilling, Thomas. / Gene expression analysis of nuclear factor I-A deficient mice indicates delayed brain maturation. In: Genome biology. 2007 ; Vol. 8, No. 5.
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Gene expression analysis of nuclear factor I-A deficient mice indicates delayed brain maturation. / Wong, Yong; Schulze, Christian; Streichert, Thomas; Gronostajski, Richard M.; Camartin, Melitta; Tilling, Thomas.

In: Genome biology, Vol. 8, No. 5, R72, 02.05.2007.

Research output: Contribution to journalArticle

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