TY - JOUR
T1 - Generation and analysis of expressed sequence tags from the tender shoots cDNA library of tea plant (Camellia sinensis)
AU - Chen, Liang
AU - Zhao, Li Ping
AU - Gao, Qi Kang
N1 - Funding Information:
This work was supported in part by the National Natural Science Foundation of China (30070486) and, the Zhejiang Provincial Key Science and Technology Project (2004C22033). We are grateful to Professor Zongmao Chen (the Academician of Chinese Academy of Engineering) in the TRICAAS, Drs. Jianyun Ruan at Kiel University, Germany and Jin Su at Cornell University, USA for their critical reading and two anonymous referees for their constructive comments.
PY - 2005/2
Y1 - 2005/2
N2 - Tea possesses a great value due to its popularity as a drink and as a source of secondary metabolic products. Up to now, the genomic information and the gene expression patterns of tea plant (Camellia sinensis) have not been studied in detail. In this report, a total of 4320 cDNA clones from a cDNA library of tender shoots from tea plant were randomly sequenced and analyzed. We generated a total of 1684 high-quality EST sequences (average 478 bp in length), among which 1200 ESTs had significant homology with the sequences in NCBI non-redundant protein database by BlastX analysis. The database-matched ESTs were further classified into 12 putative cellular roles based on the functional categories established for Arabidopsis, i.e., metabolism-related category (corresponding 8.3% of total BlastX matched ESTs), energy (26.4%), cell growth and division (3.8%), transcription (3.9%), protein synthesis (17.9%), protein destination and storage (1.7%), transporters (4.7%), cell structure (10.7%), signal transduction (5.4%), disease and defence (9.2%), secondary metabolism (4.8%), and unclear classification (3.4%). One thousand and eight ESTs of known or putative functions could be assigned to 304 functional genes and they were grouped to highly abundant (10%), moderate abundant (30%) and low abundant (60%) expression, respectively. Our study provides an overview of mRNA expression profile and a diverse genetic resource for tea plant research.
AB - Tea possesses a great value due to its popularity as a drink and as a source of secondary metabolic products. Up to now, the genomic information and the gene expression patterns of tea plant (Camellia sinensis) have not been studied in detail. In this report, a total of 4320 cDNA clones from a cDNA library of tender shoots from tea plant were randomly sequenced and analyzed. We generated a total of 1684 high-quality EST sequences (average 478 bp in length), among which 1200 ESTs had significant homology with the sequences in NCBI non-redundant protein database by BlastX analysis. The database-matched ESTs were further classified into 12 putative cellular roles based on the functional categories established for Arabidopsis, i.e., metabolism-related category (corresponding 8.3% of total BlastX matched ESTs), energy (26.4%), cell growth and division (3.8%), transcription (3.9%), protein synthesis (17.9%), protein destination and storage (1.7%), transporters (4.7%), cell structure (10.7%), signal transduction (5.4%), disease and defence (9.2%), secondary metabolism (4.8%), and unclear classification (3.4%). One thousand and eight ESTs of known or putative functions could be assigned to 304 functional genes and they were grouped to highly abundant (10%), moderate abundant (30%) and low abundant (60%) expression, respectively. Our study provides an overview of mRNA expression profile and a diverse genetic resource for tea plant research.
KW - Camellia sinensis
KW - EST
KW - Tender shoots
KW - cDNA library
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U2 - 10.1016/j.plantsci.2004.08.009
DO - 10.1016/j.plantsci.2004.08.009
M3 - Article
AN - SCOPUS:10344221610
SN - 0168-9452
VL - 168
SP - 359
EP - 363
JO - Plant Science
JF - Plant Science
IS - 2
ER -