Generation and analysis of expressed sequence tags from the tender shoots cDNA library of tea plant (Camellia sinensis)

Liang Chen, Li Ping Zhao, Qi Kang Gao

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Tea possesses a great value due to its popularity as a drink and as a source of secondary metabolic products. Up to now, the genomic information and the gene expression patterns of tea plant (Camellia sinensis) have not been studied in detail. In this report, a total of 4320 cDNA clones from a cDNA library of tender shoots from tea plant were randomly sequenced and analyzed. We generated a total of 1684 high-quality EST sequences (average 478 bp in length), among which 1200 ESTs had significant homology with the sequences in NCBI non-redundant protein database by BlastX analysis. The database-matched ESTs were further classified into 12 putative cellular roles based on the functional categories established for Arabidopsis, i.e., metabolism-related category (corresponding 8.3% of total BlastX matched ESTs), energy (26.4%), cell growth and division (3.8%), transcription (3.9%), protein synthesis (17.9%), protein destination and storage (1.7%), transporters (4.7%), cell structure (10.7%), signal transduction (5.4%), disease and defence (9.2%), secondary metabolism (4.8%), and unclear classification (3.4%). One thousand and eight ESTs of known or putative functions could be assigned to 304 functional genes and they were grouped to highly abundant (10%), moderate abundant (30%) and low abundant (60%) expression, respectively. Our study provides an overview of mRNA expression profile and a diverse genetic resource for tea plant research.

Original languageEnglish (US)
Pages (from-to)359-363
Number of pages5
JournalPlant Science
Volume168
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Keywords

  • Camellia sinensis
  • EST
  • Tender shoots
  • cDNA library

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