Generation and characterization of antibodies to sulfated glucuronyl glycolipids in Lewis rats

Amjad A. Ilyas, Zei Wei Chen, John W. Prineas

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Antibodies to sulfated glucuronyl glycolipids (SGGLs) have been reported in sera of patients with peripheral neuropathies including patients with IgM gammopathy. However, the role of anti-SGGL antibodies in the pathogenesis of neuropathy remains unclear. In order to study the role of antibodies to SGGLs in the pathogenesis of neuropathy, Lewis female rats were injected with purified SGPG mixed with keyhole limpet hemocyanin (KLH) and emulsified with equal amount of complete Freund's adjuvant. High titer anti-SGPG antibodies were detected by ELISA in sera of all rats inoculated with SGPG. All anti-SGPG antibodies cross-reacted with human myelin-associated glycoprotein (MAG). None of the sensitized rats exhibited clinical signs of neuropathy. Histological examination showed that there was no demyelination or axonal damage in peripheral nerves. Our data demonstrate that SGPG is a highly immunogenic glycolipid but high titer antibodies against it do not produce an experimental autoimmune neuropathy in Lewis rats.

Original languageEnglish (US)
Pages (from-to)54-58
Number of pages5
JournalJournal of Neuroimmunology
Volume127
Issue number1-2
DOIs
StatePublished - 2002

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Keywords

  • Antibodies
  • Glycolipids
  • Myelin-associated glycoprotein (MAG)
  • Peripheral neuropathy
  • SGPG

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