Genetic analysis of BRCA1 function in a defined tumor cell line

Ralph Scully, Shridar Ganesan, Katerina Vlasakova, Junjie Chen, Merav Socolovsky, David M. Livingston

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Abstract

Retrovirally expressed, wild-type BRCA1 decreased the γ radiation (IR) sensitivity and increased the efficiency of double-strand DNA break repair (DSBR) of the BRCA1(-/-) human breast cancer line, HCC1937. It also reduced its susceptibility to DSB generation by IR. By contrast, multiple, clinically validated, missense mutant BRCA1 products were nonfunctional in these assays. These data constitute the basis for a BRCA1 functional assay and suggest that efficient repair of double-strand DNA breaks is linked to BRCA1 tumor suppression function.

Original languageEnglish (US)
Pages (from-to)1093-1099
Number of pages7
JournalMolecular cell
Volume4
Issue number6
DOIs
Publication statusPublished - Dec 1999

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Scully, R., Ganesan, S., Vlasakova, K., Chen, J., Socolovsky, M., & Livingston, D. M. (1999). Genetic analysis of BRCA1 function in a defined tumor cell line. Molecular cell, 4(6), 1093-1099. https://doi.org/10.1016/S1097-2765(00)80238-5