Genetic analysis of glufosinate resistance in crosses between transformed rice (Oryza sativa) and red rice (Oryza sativa)

Sujatha Sankula, Michael P. Braverman, James H. Oard

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Reciprocal controlled crosses were made in the greenhouse between Gulfmont rice transformed with the bialaphos resistance (BAR) gene and red rice and BAR-transformed Koshihikari rice and red rice to assess the inheritance of glufosinate resistance. All F1 plants were resistant to 2.2 kg ai/ha glufosinate. Ammonia accumulation as a measure of glufosinate resistance in the F1 hybrids was assayed at 4 and 8 days after treatment (DAT). Ammonia accumulation in hybrids 4 DAT was similar to glufosinate treated, transformed rice, while treated nontransformed plants accumulated 14 to 23 times more ammonia compared with the hybrids. The nature of inheritance of glufosinate resistance in F2 rice plants was studied by a glufosinate dip test, a spray test, and ammonia assay. All three tests confirmed that glufosinate resistance, as influenced by the BAR gene, segregated in a 3 (resistant): 1 (susceptible) ratio.

Original languageEnglish (US)
Pages (from-to)209-214
Number of pages6
JournalWeed Technology
Volume12
Issue number2
DOIs
StatePublished - 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Keywords

  • Ammonia assay
  • BAR gene
  • Herbicide-resistant crops
  • Inheritance
  • ORYSA
  • Transformed rice

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