Genetic analysis of opaque2 modifier loci in quality protein maize

David R. Holding, Brenda G. Hunter, Taijoon Chung, Bryan C. Gibbon, Clark F. Ford, Arvind K. Bharti, Joachim Messing, Bruce R. Hamaker, Brian A. Larkins

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


Quality protein maize (QPM) was created by selecting genetic modifiers that convert the starchy endosperm of an opaque2 (o2) mutant to a hard, vitreous phenotype. Genetic analysis has shown that there are multiple, unlinked o2 modifiers (Opm), but their identity and mode of action are unknown. Using two independently developed QPM lines, we mapped several major Opm QTLs to chromosomes 1, 7 and 9. A microarray hybridization performed with RNA obtained from true breeding o2 progeny with vitreous and opaque kernel phenotypes identified a small group of differentially expressed genes, some of which map at or near the Opm QTLs. Several of the genes are associated with ethylene and ABA signaling and suggest a potential linkage of o2 endosperm modification with programmed cell death.

Original languageEnglish (US)
Pages (from-to)157-170
Number of pages14
JournalTheoretical and Applied Genetics
Issue number2
StatePublished - Jul 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics


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