Genetic analysis of opaque2 modifier loci in quality protein maize

David R. Holding; Brenda G. Hunter; Taijoon Chung; Bryan C. Gibbon; Clark F. Ford; Arvind K. Bharti; Joachim Messing; Bruce R. Hamaker; Brian A. Larkins

doi:10.1007/s00122-008-0762-y

Genetic analysis of opaque2 modifier loci in quality protein maize

David R. Holding, Brenda G. Hunter, Taijoon Chung, Bryan C. Gibbon, Clark F. Ford, Arvind K. Bharti, Joachim Messing, Bruce R. Hamaker, Brian A. Larkins

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Abstract

Quality protein maize (QPM) was created by selecting genetic modifiers that convert the starchy endosperm of an opaque2 (o2) mutant to a hard, vitreous phenotype. Genetic analysis has shown that there are multiple, unlinked o2 modifiers (Opm), but their identity and mode of action are unknown. Using two independently developed QPM lines, we mapped several major Opm QTLs to chromosomes 1, 7 and 9. A microarray hybridization performed with RNA obtained from true breeding o2 progeny with vitreous and opaque kernel phenotypes identified a small group of differentially expressed genes, some of which map at or near the Opm QTLs. Several of the genes are associated with ethylene and ABA signaling and suggest a potential linkage of o2 endosperm modification with programmed cell death.

Original language	English (US)
Pages (from-to)	157-170
Number of pages	14
Journal	Theoretical and Applied Genetics
Volume	117
Issue number	2
DOIs	https://doi.org/10.1007/s00122-008-0762-y
State	Published - Jul 2008

All Science Journal Classification (ASJC) codes

Biotechnology
Agronomy and Crop Science
Genetics

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title = "Genetic analysis of opaque2 modifier loci in quality protein maize",

abstract = "Quality protein maize (QPM) was created by selecting genetic modifiers that convert the starchy endosperm of an opaque2 (o2) mutant to a hard, vitreous phenotype. Genetic analysis has shown that there are multiple, unlinked o2 modifiers (Opm), but their identity and mode of action are unknown. Using two independently developed QPM lines, we mapped several major Opm QTLs to chromosomes 1, 7 and 9. A microarray hybridization performed with RNA obtained from true breeding o2 progeny with vitreous and opaque kernel phenotypes identified a small group of differentially expressed genes, some of which map at or near the Opm QTLs. Several of the genes are associated with ethylene and ABA signaling and suggest a potential linkage of o2 endosperm modification with programmed cell death.",

author = "Holding, {David R.} and Hunter, {Brenda G.} and Taijoon Chung and Gibbon, {Bryan C.} and Ford, {Clark F.} and Bharti, {Arvind K.} and Joachim Messing and Hamaker, {Bruce R.} and Larkins, {Brian A.}",

note = "Funding Information: The research described in this manuscript was supported by grants from the USDA (CSREES 2004-35301-14537 to BAL) and DOE (# DE-FG05-95ER20194 to JM). We thank Roberto Lizzaraga-Guerra for creating the genetic materials that were used in this analysis, and acknowledge his contribution to the QTL marker analysis. The authors owe a special thanks to Dr. William Bickel in the Physics Department at the University of Arizona, who tested a variety of procedures for measuring kernel vitreousness, and to Max Feldman for technical support. Authors also thank Tim Helentjaris and John Klingler for reviewing and commenting on the manuscript.",

year = "2008",

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doi = "10.1007/s00122-008-0762-y",

language = "English (US)",

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journal = "Theoretical And Applied Genetics",

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AU - Holding, David R.

AU - Hunter, Brenda G.

AU - Chung, Taijoon

AU - Gibbon, Bryan C.

AU - Ford, Clark F.

AU - Bharti, Arvind K.

AU - Messing, Joachim

AU - Hamaker, Bruce R.

AU - Larkins, Brian A.

N1 - Funding Information: The research described in this manuscript was supported by grants from the USDA (CSREES 2004-35301-14537 to BAL) and DOE (# DE-FG05-95ER20194 to JM). We thank Roberto Lizzaraga-Guerra for creating the genetic materials that were used in this analysis, and acknowledge his contribution to the QTL marker analysis. The authors owe a special thanks to Dr. William Bickel in the Physics Department at the University of Arizona, who tested a variety of procedures for measuring kernel vitreousness, and to Max Feldman for technical support. Authors also thank Tim Helentjaris and John Klingler for reviewing and commenting on the manuscript.

PY - 2008/7

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Genetic analysis of opaque2 modifier loci in quality protein maize

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